| Literature DB >> 9876127 |
S J Dodd1, M Williams, J P Suhan, D S Williams, A P Koretsky, C Ho.
Abstract
This study reports the detection of single mammalian cells, specifically T cells (T lymphocytes) labeled with dextran-coated superparamagnetic iron oxide particles, using magnetic resonance microscopy. Size amplification due to sequestration of the superparamagnetic particles in vacuoles enhances contrast in localized areas in high-resolution magnetic resonance imaging. Magnetic resonance images of samples containing differing concentrations of T cells embedded in 3% gelatin show a number of dark regions due to the superparamagnetic iron oxide particles, consistent with the number predicted by transmission electron microscopy. Colabeling of T cell samples with a fluorescent dye leads to strong correlations between magnetic resonance and fluorescence microscopic images, showing the presence of the superparamagnetic iron oxide particles at the cell site. This result lays the foundation for our approach to tracking the movement of a specific cell type in live animals and humans.Entities:
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Year: 1999 PMID： 9876127 PMCID： PMC1302504 DOI： 10.1016/S0006-3495(99)77182-1
Source DB: PubMed Journal: Biophys J ISSN： 0006-3495 Impact factor: 4.033