Literature DB >> 9866678

An improved sample packing device for rapid freeze-trap electron paramagnetic resonance spectroscopy kinetic measurements.

A L Tsai1, V Berka, R J Kulmacz, G Wu, G Palmer.   

Abstract

A new method has been developed for sample packing in rapid freeze-quench electron paramagnetic resonance spectroscopy (EPR) kinetic experiments. Sample particles freeze-quenched in chilled isopentane are filtered under pressure through a stainless steel funnel attached to an EPR tube fitted with a porous disk at its bottom. Isopentane exits through the porous disk and the sample particles can be transferred essentially quantitatively into the receiving EPR tube. This device provides a more predictable, reproducible, and time-saving method for sample packing, enables use of a wider range of flow velocity, and allows efficient use of valuable reactants.

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Year:  1998        PMID： 9866678     DOI： 10.1006/abio.1998.2774

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  11 in total

1.  Mechanism of binding of NO to soluble guanylyl cyclase: implication for the second NO binding to the heme proximal site.

Authors:  Emil Martin; Vladimir Berka; Iraida Sharina; Ah-Lim Tsai
Journal:  Biochemistry       Date:  2012-03-19       Impact factor: 3.162

2.  Oxyferryl heme and not tyrosyl radical is the likely culprit in prostaglandin H synthase-1 peroxidase inactivation.

Authors:  Gang Wu; Corina E Rogge; Jinn-Shyan Wang; Richard J Kulmacz; Graham Palmer; Ah-Lim Tsai
Journal:  Biochemistry       Date:  2007-01-16       Impact factor: 3.162

3.  Design, Implementation, Simulation, and Visualization of a Highly Efficient RIM Microfluidic Mixer for Rapid Freeze-Quench of Biological Samples.

Authors:  Bryan Schmidt; Goher Mahmud; Siowling Soh; Sun Hee Kim; Taylor Page; Thomas V O'Halloran; Bartosz A Grzybowski; Brian M Hoffman
Journal:  Appl Magn Reson       Date:  2011-02-11       Impact factor: 0.831

4.  Role of Tyr348 in Tyr385 radical dynamics and cyclooxygenase inhibitor interactions in prostaglandin H synthase-2.

Authors:  Corina E Rogge; Bryant Ho; Wen Liu; Richard J Kulmacz; Ah-Lim Tsai
Journal:  Biochemistry       Date:  2006-01-17       Impact factor: 3.162

5.  Highly efficient conversion of superoxide to oxygen using hydrophilic carbon clusters.

Authors:  Errol L G Samuel; Daniela C Marcano; Vladimir Berka; Brittany R Bitner; Gang Wu; Austin Potter; Roderic H Fabian; Robia G Pautler; Thomas A Kent; Ah-Lim Tsai; James M Tour
Journal:  Proc Natl Acad Sci U S A       Date:  2015-02-09       Impact factor: 11.205

6.  Cyclooxygenase reaction mechanism of prostaglandin H synthase from deuterium kinetic isotope effects.

Authors:  Gang Wu; Jian-Ming Lü; Wilfred A van der Donk; Richard J Kulmacz; Ah-lim Tsai
Journal:  J Inorg Biochem       Date:  2011-03       Impact factor: 4.155

7.  Activation of hydrogen peroxide in horseradish peroxidase occurs within approximately 200 micro s observed by a new freeze-quench device.

Authors:  Motomasa Tanaka; Koji Matsuura; Shiro Yoshioka; Satoshi Takahashi; Koichiro Ishimori; Hiroshi Hori; Isao Morishima
Journal:  Biophys J       Date:  2003-03       Impact factor: 4.033

8.  Characterization of the peroxidase mechanism upon reaction of prostacyclin synthase with peracetic acid. Identification of a tyrosyl radical intermediate.

Authors:  Hui-Chun Yeh; Gary J Gerfen; Jinn-Shyan Wang; Ah-Lim Tsai; Lee-Ho Wang
Journal:  Biochemistry       Date:  2009-02-10       Impact factor: 3.162

9.  Comparison of oxygen-induced radical intermediates in iNOS oxygenase domain with those from nNOS and eNOS.

Authors:  Vladimír Berka; Wen Liu; Gang Wu; Ah-Lim Tsai
Journal:  J Inorg Biochem       Date:  2014-06-27       Impact factor: 4.155

10.  Peroxide-induced radical formation at TYR385 and TYR504 in human PGHS-1.

Authors:  Corina E Rogge; Wen Liu; Richard J Kulmacz; Ah-Lim Tsai
Journal:  J Inorg Biochem       Date:  2009-04-17       Impact factor: 4.155
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