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Literature DB >> 986398

A modified procedure for the isolation of a pore complex-lamina fraction from rat liver nuclei.

Abstract

A modified procedure for the isolation of a nuclear pore complex-lamina fraction from rat liver nuclei is described. Evidence is provided that the isolated lamina, a 150-A thick, proteinaceous structure, apposes the inner nuclear envelope membrane, connecting nuclear pore complexes and surrounding the entire nucleus.

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Year: 1976 PMID： 986398 PMCID： PMC2109848 DOI： 10.1083/jcb.70.3.581

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

16 in total

1. A simple method for the isolation and purification of total lipides from animal tissues.

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4. A method for the rapid isolation of nuclear membranes from rat liver. Characterisation of the membrane preparation and its associated DNA polymerase.

Authors: R R Kay; D Fraser; I R Johnston
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10. Induction of a massive endoplasmic reticulum and perinuclear space expansion by expression of lamin B receptor mutants and the related sterol reductases TM7SF2 and DHCR7.

Authors: Monika Zwerger; Thorsten Kolb; Karsten Richter; Iakowos Karakesisoglou; Harald Herrmann
Journal: Mol Biol Cell Date: 2009-11-25 Impact factor: 4.138

A modified procedure for the isolation of a pore complex-lamina fraction from rat liver nuclei.

1. A simple method for the isolation and purification of total lipides from animal tissues.

2. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

3. Protein measurement with the Folin phenol reagent.

4. A method for the rapid isolation of nuclear membranes from rat liver. Characterisation of the membrane preparation and its associated DNA polymerase.

5. A negative staining study of human erythrocyte ghosts and rat liver nuclear membranes.

6. Identification of a nuclear protein matrix.

7. A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY.

8. Improvements in epoxy resin embedding methods.

9. Staining of tissue sections for electron microscopy with heavy metals.

10. Fractionation of the nucleus by divalent cations. Isolation of nuclear membranes.

1. Purification of the vertebrate nuclear pore complex by biochemical criteria.

2. In vivo and in vitro interaction between human transcription factor MOK2 and nuclear lamin A/C.

3. Isolation of chromatin DNA tightly bound to the nuclear envelope of HeLa cells.

4. Preparation and characterization of nuclear-envelope vesicles from rat liver nuclei.

Review 5. Introducing intermediate filaments: from discovery to disease.

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7. cDNA cloning and functional characterization of a meiosis-specific protein (MNS1) with apparent nuclear association.

8. Myopathic lamin mutations impair nuclear stability in cells and tissue and disrupt nucleo-cytoskeletal coupling.

9. Effect of salt-treatment on manually isolated polytene chromosomes from Chironomus tentans.

10. Induction of a massive endoplasmic reticulum and perinuclear space expansion by expression of lamin B receptor mutants and the related sterol reductases TM7SF2 and DHCR7.