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Literature DB >> 4120072

Fractionation of the nucleus by divalent cations. Isolation of nuclear membranes.

Abstract

0.3-0.5 M MgCl(2) was used to disassemble nuclei and to isolate by a single centrifugation in less than 3 hr a nuclear envelope fraction in 55-60% yield as assessed by phospholipid recovery. Its gross chemical composition was determined and its morphology was studied electron microscopically by sectioning, freeze etching, and negative staining procedures.

Entities: Chemical

Mesh：

Substances：

Year: 1972 PMID： 4120072 PMCID： PMC2108744 DOI： 10.1083/jcb.55.1.104

Source DB: PubMed Journal: J Cell Biol ISSN： 0021-9525 Impact factor: 10.539

19 in total

1. Subunits in chloroplast lamellae.

Authors: D Branton; R B Park
Journal: J Ultrastruct Res Date: 1967-08

2. Isolation, morphology, and composition of the nuclear membrane from rat liver.

Authors: D M Kashnig; C B Kasper
Journal: J Biol Chem Date: 1969-07-25 Impact factor: 5.157

3. Isolation and biochemical characteristics of the nuclear envelope.

Authors: I B Zbarsky; K A Perevoshchikova; L N Delektorskaya; V V Delektorsky
Journal: Nature Date: 1969-01-18 Impact factor: 49.962

4. Fracture faces of frozen membranes.

Authors: D Branton
Journal: Proc Natl Acad Sci U S A Date: 1966-05 Impact factor: 11.205

5. The occurrence of cytochromes in the membranous structures of calf thymus nuclei.

Authors: K Ueda; T Matsuura; N Date; K Kawai
Journal: Biochem Biophys Res Commun Date: 1969-02-07 Impact factor: 3.575

6. The structure of pores in isolated pea nuclei.

Authors: B Y Yoo; S T Bayley
Journal: J Ultrastruct Res Date: 1967-06

7. Controlled proteolysis of nascent polypeptides in rat liver cell fractions. I. Location of the polypeptides within ribosomes.

Authors: G Blobel; D D Sabatini
Journal: J Cell Biol Date: 1970-04 Impact factor: 10.539

8. Cell junctions in amphibian skin.

Authors: M G Farquhar; G E Palade
Journal: J Cell Biol Date: 1965-07 Impact factor: 10.539

9. Controlled proteolysis of nascent polypeptides in rat liver cell fractions. II. Location of the polypeptides in rough microsomes.

Authors: D D Sabatini; G Blobel
Journal: J Cell Biol Date: 1970-04 Impact factor: 10.539

10. Octagonal nuclear pores.

Authors: J G Gall
Journal: J Cell Biol Date: 1967-02 Impact factor: 10.539

17 in total

1. Isolation of nuclear pore complexes in association with a lamina.

Authors: R P Aaronson; G Blobel
Journal: Proc Natl Acad Sci U S A Date: 1975-03 Impact factor: 11.205

2. The mechanism of a nuclear pore assembly: a molecular biophysics view.

Authors: Vasily V Kuvichkin
Journal: J Membr Biol Date: 2011-06-16 Impact factor: 1.843

3. Nuclear pores. Can they expand and contrast to regulate nucleocytoplasmic exchange?

Authors: N J Severs; E G Jordan
Journal: Experientia Date: 1978-08-15

Review 4. The Quest for the Blueprint of the Nuclear Pore Complex.

Authors: Joseph S Glavy
Journal: Protein J Date: 2019-08 Impact factor: 2.371

5. Localization of oxidized nocotinamide--adenine dinucleotide glycohydrolase in the mouse liver nuclear envelope.

Authors: P Tamulevicius; C Streffer; O Roscic; E Hubert
Journal: Biochem J Date: 1979-02-15 Impact factor: 3.857

6. A 41-42 bp tandemly repeated sequence isolated from nuclear envelopes of chicken erythrocytes is located predominantly on microchromosomes.

Authors: M A Matzke; F Varga; H Berger; J Schernthaner; D Schweizer; B Mayr; A J Matzke
Journal: Chromosoma Date: 1990-04 Impact factor: 4.316

7. Insulin regulation of protein phosphorylation in isolated rat liver nuclear envelopes: potential relationship to mRNA metabolism.

Authors: F Purrello; D B Burnham; I D Goldfine
Journal: Proc Natl Acad Sci U S A Date: 1983-03 Impact factor: 11.205