Literature DB >> 9859215

Coat protein-mediated resistance to pea enation mosaic virus in transgenic Pisum sativum L.

G M Chowrira1, T D Cavileer, S K Gupta, P F Lurquin, P H Berger.   

Abstract

Pea (Pisum sativum L.) plants were transformed in planta by injection/electroporation of axillary meristems with a chimeric pea enation mosaic virus (PEMV) coat protein gene contruct. R1 progenies of these plants were shown to harbor the transgene by polymerase chain reaction (PCR) and genomic Southern analysis, while transgene expression was demonstrated by western blot analysis. Transgenic R2, R3 and R4 plants displayed delayed or transient PEMV multiplication and attenuated symptoms as compared to control inoculated individuals.

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Year:  1998        PMID： 9859215     DOI： 10.1023/a:1008874129407

Source DB:  PubMed          Journal:  Transgenic Res        ISSN： 0962-8819            Impact factor:   2.788


  13 in total

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Authors:  P. Meyer; H. Saedler
Journal:  Annu Rev Plant Physiol Plant Mol Biol       Date:  1996-06

2.  Transgenic grain legumes obtained by in planta electroporation-mediated gene transfer.

Authors:  G M Chowrira; V Akella; P E Fuerst; P F Lurquin
Journal:  Mol Biotechnol       Date:  1996-04       Impact factor: 2.695

3.  Design and construction of a versatile system for the expression of foreign genes in plants.

Authors:  C L Schardl; A D Byrd; G Benzion; M A Altschuler; D F Hildebrand; A G Hunt
Journal:  Gene       Date:  1987       Impact factor: 3.688

4.  Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses.

Authors:  M F Clark; A N Adams
Journal:  J Gen Virol       Date:  1977-03       Impact factor: 3.891

5.  Transformation and Regeneration of Two Cultivars of Pea (Pisum sativum L.).

Authors:  H. E. Schroeder; A. H. Schotz; T. Wardley-Richardson; D. Spencer; TJV. Higgins
Journal:  Plant Physiol       Date:  1993-03       Impact factor: 8.340

6.  Viral RNA as a potential target for two independent mechanisms of replicase-mediated resistance against cucumber mosaic virus.

Authors:  K H Hellwald; P Palukaitis
Journal:  Cell       Date:  1995-12-15       Impact factor: 41.582

7.  The chimeric nature of the genome of pea enation mosaic virus: the independent replication of RNA 2.

Authors:  S A Demler; D G Rucker; G A de Zoeten
Journal:  J Gen Virol       Date:  1993-01       Impact factor: 3.891

8.  Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene.

Authors:  P P Abel; R S Nelson; B De; N Hoffmann; S G Rogers; R T Fraley; R N Beachy
Journal:  Science       Date:  1986-05-09       Impact factor: 47.728

9.  A maize protein associated with the G-box binding complex has homology to brain regulatory proteins.

Authors:  N C de Vetten; G Lu; R J Feri
Journal:  Plant Cell       Date:  1992-10       Impact factor: 11.277

10.  Combinatorial and synergistic properties of CaMV 35S enhancer subdomains.

Authors:  P N Benfey; L Ren; N H Chua
Journal:  EMBO J       Date:  1990-06       Impact factor: 11.598
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