PURPOSE: To compare the amount of ascorbic acid in the corneal epithelium of various species to unveil possible differences between diurnal and nocturnal mammals. METHODS: Ascorbic acid was determined by high-performance liquid chromatography, using an LC-10 system (Shimadzu, Kyoto, Japan). RESULTS: Diurnal animals show a higher ascorbate concentration in the corneal epithelium than nocturnal animals. Ascorbate concentration is higher in the corneal epithelium than in the matching aqueous humor in diurnal and nocturnal species. The highest ascorbate concentration is found in the corneal epithelium of the reindeer. CONCLUSIONS: Ascorbate level in the corneal epithelium seems to vary in accordance with ambient radiation exposure of the respective species, just as in the aqueous humor. Both phenomena are regarded as environmental adaptations, and the ascorbic acid is suggested as protecting against photokeratitis and as acting as an ultraviolet filter for internal eye structures.
PURPOSE: To compare the amount of ascorbic acid in the corneal epithelium of various species to unveil possible differences between diurnal and nocturnal mammals. METHODS:Ascorbic acid was determined by high-performance liquid chromatography, using an LC-10 system (Shimadzu, Kyoto, Japan). RESULTS: Diurnal animals show a higher ascorbate concentration in the corneal epithelium than nocturnal animals. Ascorbate concentration is higher in the corneal epithelium than in the matching aqueous humor in diurnal and nocturnal species. The highest ascorbate concentration is found in the corneal epithelium of the reindeer. CONCLUSIONS:Ascorbate level in the corneal epithelium seems to vary in accordance with ambient radiation exposure of the respective species, just as in the aqueous humor. Both phenomena are regarded as environmental adaptations, and the ascorbic acid is suggested as protecting against photokeratitis and as acting as an ultraviolet filter for internal eye structures.
Authors: Natalie Lassen; J Bronwyn Bateman; Tia Estey; Jer R Kuszak; David W Nees; Joram Piatigorsky; Gregg Duester; Brian J Day; Jie Huang; Lisa M Hines; Vasilis Vasiliou Journal: J Biol Chem Date: 2007-06-13 Impact factor: 5.157
Authors: Sally Hayes; Thamara A Cafaro; Patrycja J Boguslawska; Christina S Kamma-Lorger; Craig Boote; Jonathan Harris; Robert Young; Jennifer Hiller; Nicholas Terrill; Keith M Meek; Horacio M Serra Journal: Mol Vis Date: 2011-11-26 Impact factor: 2.367
Authors: Nan Ma; Carla Siegfried; Miyuki Kubota; Jie Huang; Ying Liu; Margaret Liu; Belinda Dana; Andrew Huang; David Beebe; Hong Yan; Ying-Bo Shui Journal: Invest Ophthalmol Vis Sci Date: 2016-06-01 Impact factor: 4.799