OBJECTIVE: To explore the impact of eye closure and soft contact lens extended wear (SCLEW) on epithelial permeability to fluorescein (Pdc). DESIGN: A prospective cohort study. PARTICIPANTS: Thirty-one noncontact lens (CL) wearers participated. INTERVENTION: The effects of eye closure on Pdc were evaluated by comparing morning (AM) and afternoon (PM) measurements on non-CL subjects. The effects of SCLEW on Pdc were determined by measuring Pdc before beginning SCLEW and then after 2, 4, and 12 weeks of SCLEW. MAIN OUTCOME MEASURES: Pdc measured in the morning versus the afternoon and before versus after SCLEW was examined. RESULTS: Analyses of Pdc were done using the natural logarithm (ln). The mean +/- standard error (SE) ln(Pdc) measured in the AM versus PM on 16 non-CL wearers did not differ significantly (-2.56+/-0.16 vs. -2.69+/-0.15, respectively; P = 0.46). In contrast, the mean +/- SE ln(Pdc) in 15 subjects after 2 (-1.73+/-0.183, P < 0.001), 4 (- 1.59+/-0.188, P < 0.001), and 12 weeks (-1.99+/-0.206, P = 0.02) of SCLEW was substantially greater than that measured before lens wear (-2.42+/-0.159 ln(nm/sec)). Interestingly, the mean+/-SE ln(Pdc) measured in the afternoon on seven subjects after 13 weeks of SCLEW was similar to their average baseline ln(Pdc) (-2.62+/-0.27 vs. -2.52+/-0.243, respectively; P = 0.54). Further analysis showed that Pdc was highest in the morning and decreased approximately 12.5%/hour (P < 0.001) during the day. CONCLUSIONS: Sleeping without CLs does not appear to alter Pdc; however, 2 weeks of SCLEW caused a 99% increase in permeability without observable changes by slit-lamp examination. Increases in Pdc appear greatest in the morning after SCLEW and then decrease exponentially during the day. Whether changes in Pdc will predict CL-associated keratopathy needs further study.
OBJECTIVE: To explore the impact of eye closure and soft contact lens extended wear (SCLEW) on epithelial permeability to fluorescein (Pdc). DESIGN: A prospective cohort study. PARTICIPANTS: Thirty-one noncontact lens (CL) wearers participated. INTERVENTION: The effects of eye closure on Pdc were evaluated by comparing morning (AM) and afternoon (PM) measurements on non-CL subjects. The effects of SCLEW on Pdc were determined by measuring Pdc before beginning SCLEW and then after 2, 4, and 12 weeks of SCLEW. MAIN OUTCOME MEASURES: Pdc measured in the morning versus the afternoon and before versus after SCLEW was examined. RESULTS: Analyses of Pdc were done using the natural logarithm (ln). The mean +/- standard error (SE) ln(Pdc) measured in the AM versus PM on 16 non-CL wearers did not differ significantly (-2.56+/-0.16 vs. -2.69+/-0.15, respectively; P = 0.46). In contrast, the mean +/- SE ln(Pdc) in 15 subjects after 2 (-1.73+/-0.183, P < 0.001), 4 (- 1.59+/-0.188, P < 0.001), and 12 weeks (-1.99+/-0.206, P = 0.02) of SCLEW was substantially greater than that measured before lens wear (-2.42+/-0.159 ln(nm/sec)). Interestingly, the mean+/-SE ln(Pdc) measured in the afternoon on seven subjects after 13 weeks of SCLEW was similar to their average baseline ln(Pdc) (-2.62+/-0.27 vs. -2.52+/-0.243, respectively; P = 0.54). Further analysis showed that Pdc was highest in the morning and decreased approximately 12.5%/hour (P < 0.001) during the day. CONCLUSIONS: Sleeping without CLs does not appear to alter Pdc; however, 2 weeks of SCLEW caused a 99% increase in permeability without observable changes by slit-lamp examination. Increases in Pdc appear greatest in the morning after SCLEW and then decrease exponentially during the day. Whether changes in Pdc will predict CL-associated keratopathy needs further study.
Authors: Aaron B Sullivan; K P Connie Tam; Matteo M E Metruccio; David J Evans; Suzanne M J Fleiszig Journal: Infect Immun Date: 2015-02-09 Impact factor: 3.441
Authors: Thao N Yeh; Harry M Green; Yixiu Zhou; Julie Pitts; Britney Kitamata-Wong; Sophia Lee; Shiyin L Wang; Meng C Lin Journal: Invest Ophthalmol Vis Sci Date: 2013-06-06 Impact factor: 4.799
Authors: Suzanne M J Fleiszig; Abby R Kroken; Vincent Nieto; Melinda R Grosser; Stephanie J Wan; Matteo M E Metruccio; David J Evans Journal: Prog Retin Eye Res Date: 2019-11-20 Impact factor: 21.198