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Literature DB >> 9854071

Rapid identification of clinically relevant Nocardia species to genus level by 16S rRNA gene PCR.

Abstract

Two regions of the gene coding for 16S rRNA in Nocardia species were selected as genus-specific primer sequences for a PCR assay. The PCR protocol was tested with 60 strains of clinically relevant Nocardia isolates and type strains. It gave positive results for all strains tested. Conversely, the PCR assay was negative for all tested species belonging to the most closely related genera, including Dietzia, Gordona, Mycobacterium, Rhodococcus, Streptomyces, and Tsukamurella. Besides, unlike the latter group of isolates, all Nocardia strains exhibited one MlnI recognition site but no SacI restriction site. This assay offers a specific and rapid alternative to chemotaxonomic methods for the identification of Nocardia spp. isolated from pathogenic samples.

Entities: Disease

Mesh：

Substances：

Year: 1999 PMID： 9854071 PMCID： PMC84177

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

23 in total

1. Genetic identification of members of the genus Corynebacterium at genus and species levels with 16S rDNA-targeted probes.

Authors: X G Hou; Y Kawamura; F Sultana; K Hirose; M Miyake; Y Otsuka; S Misawa; T Oguri; H Yamamoto; T Ezaki
Journal: Microbiol Immunol Date: 1997 Impact factor: 1.955

2. 16S rDNA genotyping using PCR/RFLP (restriction fragment length polymorphism) analysis among the family Vibrionaceae.

Authors: H Urakawa; K Kita-Tsukamoto; K Ohwada
Journal: FEMS Microbiol Lett Date: 1997-07-01 Impact factor: 2.742

3. Identification of Aeromonas clinical isolates by restriction fragment length polymorphism of PCR-amplified 16S rRNA genes.

Authors: N Borrell; S G Acinas; M J Figueras; A J Martínez-Murcia
Journal: J Clin Microbiol Date: 1997-07 Impact factor: 5.948

4. Identification of clinically relevant viridans group streptococci to the species level by PCR.

Authors: F Garnier; G Gerbaud; P Courvalin; M Galimand
Journal: J Clin Microbiol Date: 1997-09 Impact factor: 5.948

5. Progressive sequence alignment as a prerequisite to correct phylogenetic trees.

Authors: D F Feng; R F Doolittle
Journal: J Mol Evol Date: 1987 Impact factor: 2.395

6. A comprehensive set of sequence analysis programs for the VAX.

Authors: J Devereux; P Haeberli; O Smithies
Journal: Nucleic Acids Res Date: 1984-01-11 Impact factor: 16.971

7. Detection of Neisseria gonorrhoeae and Chlamydia trachomatis in genitourinary specimens from men and women by a coamplification PCR assay.

Authors: K A Crotchfelt; L E Welsh; D DeBonville; M Rosenstraus; T C Quinn
Journal: J Clin Microbiol Date: 1997-06 Impact factor: 5.948

8. Clinical application of PCR-restriction enzyme pattern analysis for rapid identification of aerobic actinomycete isolates.

Authors: R W Wilson; V A Steingrube; B A Brown; R J Wallace
Journal: J Clin Microbiol Date: 1998-01 Impact factor: 5.948

9. A PCR assay for identification of Enterococcus faecium.

Authors: S Cheng; F K McCleskey; M J Gress; J M Petroziello; R Liu; H Namdari; K Beninga; A Salmen; V G DelVecchio
Journal: J Clin Microbiol Date: 1997-05 Impact factor: 5.948

10. Identification of Borrelia burgdorferi sensu lato tick isolates from Slovakia by PCR typing with 16S rRNA primers.

Authors: F Mateicka; D Kozáková; P A Rosa; E Kmety
Journal: Zentralbl Bakteriol Date: 1997-10

44 in total

Review 1. Nocardiosis: review of clinical and laboratory experience.

Authors: Michael A Saubolle; Den Sussland
Journal: J Clin Microbiol Date: 2003-10 Impact factor: 5.948

2. Nocardia mexicana sp. nov., a new pathogen isolated from human mycetomas.

Authors: Verónica Rodríguez-Nava; Andrée Couble; Claudie Molinard; Horacio Sandoval; Patrick Boiron; Frédéric Laurent
Journal: J Clin Microbiol Date: 2004-10 Impact factor: 5.948

3. Nocardia ignorata, a new agent of human nocardiosis isolated from respiratory specimens in Europe and soil samples from Kuwait.

Authors: Verónica Rodríguez-Nava; Andrée Couble; Z U Khan; M Pérouse de Montclos; L Brasme; Cruz Villuendas; Claudie Molinard; Patrick Boiron; Frédéric Laurent
Journal: J Clin Microbiol Date: 2005-12 Impact factor: 5.948

4. Use of PCR-restriction enzyme pattern analysis and sequencing database for hsp65 gene-based identification of Nocardia species.

Authors: Verónica Rodríguez-Nava; Andrée Couble; Gregory Devulder; Jean-Pierre Flandrois; Patrick Boiron; Frédéric Laurent
Journal: J Clin Microbiol Date: 2006-02 Impact factor: 5.948

10. Rapid identification of Nocardia farcinica clinical isolates by a PCR assay targeting a 314-base-pair species-specific DNA fragment.

Authors: June M Brown; Kim N Pham; Michael M McNeil; Brent A Lasker
Journal: J Clin Microbiol Date: 2004-08 Impact factor: 5.948