| Literature DB >> 9835068 |
H Rosing1, M J Hillebrand, J M Jimeno, A Gómez, P Floriano, G Faircloth, R E Henrar, J B Vermorken, E Cvitkovic, A Bult, J H Beijnen.
Abstract
A method was developed for the bio-analysis of Ecteinascidin 743 (ET-743) using miniaturized liquid chromatography (LC) coupled to an electrospray ionization sample inlet (TurbolonSpray) and two quadrupole mass analyzers (LC/ESI-MS/MS). Solid-phase extraction was used as a sample pretreatment procedure. Ecteinascidin 743 is a very potent anticancer compound and is administered in microgram m-2 dosages, which demands special requirements in terms of sensitivity for the analytical method supporting clinical pharmacokinetic studies. Using conventional LC/UV, a lower limit of quantitation (LLQ) of 1 ng ml-1 plasma was reached using a 500 microliters sample volume, but LC/ESI-MS/MS permitted an LLQ of 10 pg ml-1. The latter method was found to be accurate and precise, and provided a broad linear concentration range of 0.010-2.50 ng ml-1.Entities:
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Year: 1998 PMID： 9835068 DOI： 10.1002/(SICI)1096-9888(1998110)33:11<1134::AID-JMS730>3.0.CO;2-S
Source DB: PubMed Journal: J Mass Spectrom ISSN： 1076-5174 Impact factor: 1.982