Literature DB >> 9826654

DNA end-joining catalyzed by human cell-free extracts.

P Baumann1, S C West.   

Abstract

Mammalian cells defective in DNA end-joining are highly sensitive to ionizing radiation and are immunodeficient because of a failure to complete V(D)J recombination. By using cell-free extracts prepared from human lymphoblastoid cell lines, an in vitro system for end-joining has been developed. Intermolecular ligation was found to be accurate and to depend on DNA ligase IV/Xrcc4 and requires Ku70, Ku86, and DNA-PKcs, the three subunits of the DNA-activated protein kinase DNA-PK. Because these activities are involved in the cellular resistance to x-irradiation and V(D)J recombination, the development of this in vitro system provides an important advance in the study of the mechanism of DNA end-joining in human cells.

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Year:  1998        PMID： 9826654      PMCID： PMC24327          DOI： 10.1073/pnas.95.24.14066

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  47 in total

1.  Multiple components are involved in the efficient joining of double stranded DNA breaks in human cell extracts.

Authors:  M P Fairman; A P Johnson; J Thacker
Journal:  Nucleic Acids Res       Date:  1992-08-25       Impact factor: 16.971

Review 2.  Mammalian DNA ligases.

Authors:  T Lindahl; D E Barnes
Journal:  Annu Rev Biochem       Date:  1992       Impact factor: 23.643

3.  DNA-dependent protein kinase catalytic subunit: a relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product.

Authors:  K O Hartley; D Gell; G C Smith; H Zhang; N Divecha; M A Connelly; A Admon; S P Lees-Miller; C W Anderson; S P Jackson
Journal:  Cell       Date:  1995-09-08       Impact factor: 41.582

4.  Nomenclature of human genes involved in ionizing radiation sensitivity.

Authors:  L H Thompson; P A Jeggo
Journal:  Mutat Res       Date:  1995-09       Impact factor: 2.433

5.  Impairment of V(D)J recombination in double-strand break repair mutants.

Authors:  G E Taccioli; G Rathbun; E Oltz; T Stamato; P A Jeggo; F W Alt
Journal:  Science       Date:  1993-04-09       Impact factor: 47.728

6.  Absence of a Ku-like DNA end binding activity in the xrs double-strand DNA repair-deficient mutant.

Authors:  R C Getts; T D Stamato
Journal:  J Biol Chem       Date:  1994-06-10       Impact factor: 5.157

7.  Activation of RuvC Holliday junction resolvase in vitro.

Authors:  R Shah; R J Bennett; S C West
Journal:  Nucleic Acids Res       Date:  1994-07-11       Impact factor: 16.971

8.  Ku80: product of the XRCC5 gene and its role in DNA repair and V(D)J recombination.

Authors:  G E Taccioli; T M Gottlieb; T Blunt; A Priestley; J Demengeot; R Mizuta; A R Lehmann; F W Alt; S P Jackson; P A Jeggo
Journal:  Science       Date:  1994-09-02       Impact factor: 47.728

9.  Defective DNA-dependent protein kinase activity is linked to V(D)J recombination and DNA repair defects associated with the murine scid mutation.

Authors:  T Blunt; N J Finnie; G E Taccioli; G C Smith; J Demengeot; T M Gottlieb; R Mizuta; A J Varghese; F W Alt; P A Jeggo; S P Jackson
Journal:  Cell       Date:  1995-03-10       Impact factor: 41.582

10.  DNA end-joining in extracts from human cells.

Authors:  S O Bøe; J Sodroski; D E Helland; C M Farnet
Journal:  Biochem Biophys Res Commun       Date:  1995-10-24       Impact factor: 3.575
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  111 in total

1.  Ku recruits the XRCC4-ligase IV complex to DNA ends.

Authors:  S A Nick McElhinny; C M Snowden; J McCarville; D A Ramsden
Journal:  Mol Cell Biol       Date:  2000-05       Impact factor: 4.272

2.  DNA damage-dependent nuclear dynamics of the Mre11 complex.

Authors:  O K Mirzoeva; J H Petrini
Journal:  Mol Cell Biol       Date:  2001-01       Impact factor: 4.272

Review 3.  Protein kinases as therapeutic targets.

Authors:  R Sridhar; O Hanson-Painton; D R Cooper
Journal:  Pharm Res       Date:  2000-11       Impact factor: 4.200

4.  DNA double-strand break repair in cell-free extracts from Ku80-deficient cells: implications for Ku serving as an alignment factor in non-homologous DNA end joining.

Authors:  E Feldmann; V Schmiemann; W Goedecke; S Reichenberger; P Pfeiffer
Journal:  Nucleic Acids Res       Date:  2000-07-01       Impact factor: 16.971

5.  Reconstitution of the mammalian DNA double-strand break end-joining reaction reveals a requirement for an Mre11/Rad50/NBS1-containing fraction.

Authors:  Juren Huang; William S Dynan
Journal:  Nucleic Acids Res       Date:  2002-02-01       Impact factor: 16.971

6.  Suppression of gene amplification and chromosomal DNA integration by the DNA mismatch repair system.

Authors:  C T Lin; Y L Lyu; H Xiao; W H Lin; J Whang-Peng
Journal:  Nucleic Acids Res       Date:  2001-08-15       Impact factor: 16.971

7.  Holliday junction resolution in human cells: two junction endonucleases with distinct substrate specificities.

Authors:  Angelos Constantinou; Xiao-Bo Chen; Clare H McGowan; Stephen C West
Journal:  EMBO J       Date:  2002-10-15       Impact factor: 11.598

8.  A single amino acid substitution in DNA-PKcs explains the novel phenotype of the CHO mutant, XR-C2.

Authors:  Timothy Woods; Wei Wang; Erin Convery; Abdellatif Errami; Malgorzata Z Zdzienicka; Katheryn Meek
Journal:  Nucleic Acids Res       Date:  2002-12-01       Impact factor: 16.971

9.  Gross chromosomal rearrangements and genetic exchange between nonhomologous chromosomes following BRCA2 inactivation.

Authors:  V P Yu; M Koehler; C Steinlein; M Schmid; L A Hanakahi; A J van Gool; S C West; A R Venkitaraman
Journal:  Genes Dev       Date:  2000-06-01       Impact factor: 11.361

10.  The integrin alpha2beta1 agonist, aggretin, promotes proliferation and migration of VSMC through NF-kB translocation and PDGF production.

Authors:  Ching-Hu Chung; Kuan-Ting Lin; Chien-Hsin Chang; Hui-Chin Peng; Tur-Fu Huang
Journal:  Br J Pharmacol       Date:  2009-02-23       Impact factor: 8.739
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