Literature DB >> 980019

Prenatal diagnosis of alpha-thalassemia. Clinical application of molecular hybridization.

Y W Kan, M S Golbus, A M Dozy.   

Abstract

The technic of DNA-DNA hybridization was used for prenatal diagnosis of a pregnancy at risk for homozygous alpha-thalassemia. Fibroblasts were cultured from amniotic fluid, and the number of alpha-globin genes in the DNA was quantified by hybridization with radioactive DNA complementary to alpha-globin mRNA sequences. As compared to control studies of DNA from patients with alpha-thalassemia syndromes and from unaffected subjects, the results indicated that the fetus had alpha-thalassemia-1. The diagnosis was confirmed by umbilical-cord blood studies.

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Year:  1976        PMID： 980019     DOI： 10.1056/NEJM197611182952104

Source DB:  PubMed          Journal:  N Engl J Med        ISSN： 0028-4793            Impact factor:   91.245


  17 in total

Review 1.  The new biomedical technology.

Authors:  C H Scoggin
Journal:  West J Med       Date:  1985-12

2.  Improved detection of beta-thalassaemia carriers by a two-test method.

Authors:  M Tammis-Hadjopoulos; R J Gold; U R Maag; J D Metrakos; C R Scriver
Journal:  Hum Genet       Date:  1977-10-14       Impact factor: 4.132

3.  Alpha-thalassemia mutations in adana province, southern Turkey: genotype-phenotype correlation.

Authors:  Sevcan Tug Bozdogan; Ozge Ozalp Yuregir; Nurhilal Buyukkurt; Huseyin Aslan; Zeynep Canan Ozdemir; Tomasz Gambin
Journal:  Indian J Hematol Blood Transfus       Date:  2014-06-13       Impact factor: 0.900

4.  The William Allan Memorial Award address: Thalassemia: molecular mechanism and detection.

Authors:  Y W Kan
Journal:  Am J Hum Genet       Date:  1986-01       Impact factor: 11.025

Review 5.  Antenatal genetic diagnosis: current status and future prospects.

Authors:  R J Benzie
Journal:  Can Med Assoc J       Date:  1979-03-17       Impact factor: 8.262

6.  Direct identification of sickle cell anemia by blot hybridization.

Authors:  R F Geever; L B Wilson; F S Nallaseth; P F Milner; M Bittner; J T Wilson
Journal:  Proc Natl Acad Sci U S A       Date:  1981-08       Impact factor: 11.205

7.  Prenatal prediction of alpha-thalassemia phenotype by endonuclease mapping of parental DNA.

Authors:  U P Steinbrecher; Y W Kan; N K de Leeuw
Journal:  Can Med Assoc J       Date:  1982-01-15       Impact factor: 8.262

Review 8.  The thalassemias: molecular mechanisms of human genetic disease.

Authors:  R A Spritz; B G Forget
Journal:  Am J Hum Genet       Date:  1983-05       Impact factor: 11.025

9.  Fetal gene therapy of alpha-thalassemia in a mouse model.

Authors:  Xiao-Dong Han; Chin Lin; Judy Chang; Michel Sadelain; Y W Kan
Journal:  Proc Natl Acad Sci U S A       Date:  2007-05-11       Impact factor: 11.205

Review 10.  The prevention of thalassemia.

Authors:  Antonio Cao; Yuet Wai Kan
Journal:  Cold Spring Harb Perspect Med       Date:  2013-02-01       Impact factor: 6.915
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