V Serre1, B Robaire. 1. Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec, Canada.
Abstract
OBJECTIVE: To investigate the effects of paternal age on fertility and progeny outcome using the Brown Norway rat model. DESIGN: Controlled prospective study. SETTING: McIntyre Animal Centre, McGill University, Montreal, Quebec, Canada. INTERVENTION(S): Brown Norway male rats of increasing age were mated to young Sprague-Dawley females. MAIN OUTCOME MEASURE(S): Pregnancy outcome was assessed by counting the numbers of corpora lutea, resorptions, and live fetuses on day 20 of gestation. To evaluate progeny outcome, pups were examined for external malformations and weighed daily for 2 months. RESULT(S): There were no significant changes in the numbers of resorptions, offspring, or in the incidence of external malformations. However, there was an increase in preimplantation loss (corpora lutea minus implantation sites) in litters fathered by older males. Furthermore, a significant decrease in the average fetal weight was found with increasing paternal age. A significant increase in neonatal deaths for progeny fathered by older males also was found. CONCLUSION(S): These results indicate that the quality of spermatozoa decreases as males age.
OBJECTIVE: To investigate the effects of paternal age on fertility and progeny outcome using the Brown Norway rat model. DESIGN: Controlled prospective study. SETTING: McIntyre Animal Centre, McGill University, Montreal, Quebec, Canada. INTERVENTION(S): Brown Norway male rats of increasing age were mated to young Sprague-Dawley females. MAIN OUTCOME MEASURE(S): Pregnancy outcome was assessed by counting the numbers of corpora lutea, resorptions, and live fetuses on day 20 of gestation. To evaluate progeny outcome, pups were examined for external malformations and weighed daily for 2 months. RESULT(S): There were no significant changes in the numbers of resorptions, offspring, or in the incidence of external malformations. However, there was an increase in preimplantation loss (corpora lutea minus implantation sites) in litters fathered by older males. Furthermore, a significant decrease in the average fetal weight was found with increasing paternal age. A significant increase in neonatal deaths for progeny fathered by older males also was found. CONCLUSION(S): These results indicate that the quality of spermatozoa decreases as males age.
Authors: Christopher C Oakes; Dominic J Smiraglia; Christoph Plass; Jacquetta M Trasler; Bernard Robaire Journal: Proc Natl Acad Sci U S A Date: 2003-02-06 Impact factor: 11.205
Authors: Jason R Kovac; Josephine Addai; Ryan P Smith; Robert M Coward; Dolores J Lamb; Larry I Lipshultz Journal: Asian J Androl Date: 2013-08-05 Impact factor: 3.285