BACKGROUND: Steady-state autofluorescence spectroscopy has been examined previously as a technique for distinguishing polyp types during colonoscopy. Although time-resolved methods have shown promise for tissue diagnosis in vitro, they have never been applied endoscopically. The aim of this study was to examine the feasibility of performing time-resolved autofluorescence spectroscopy in vivo and to determine the diagnostic accuracy of the technique as applied to colonic dysplasia. METHODS: A time-resolved spectrometer was used to measure the spectrally resolved transient decay of laser-induced autofluorescence emission from colonic tissue in vivo. RESULTS: Seventeen patients with 24 polyps (13 adenomatous, 11 non-adenomatous) were studied. The autofluorescence decay from adenomas was faster than that from non-adenomas. The measured decay time provided a means of distinguishing adenomas from non-adenomas with a sensitivity of 85%, a specificity of 91%, a positive predictive value of 92%, and a negative predictive value of 83%. CONCLUSIONS: Time-resolved autofluorescence spectroscopy is a promising optical diagnostic technique for determining polyp types in vivo.
BACKGROUND: Steady-state autofluorescence spectroscopy has been examined previously as a technique for distinguishing polyp types during colonoscopy. Although time-resolved methods have shown promise for tissue diagnosis in vitro, they have never been applied endoscopically. The aim of this study was to examine the feasibility of performing time-resolved autofluorescence spectroscopy in vivo and to determine the diagnostic accuracy of the technique as applied to colonic dysplasia. METHODS: A time-resolved spectrometer was used to measure the spectrally resolved transient decay of laser-induced autofluorescence emission from colonic tissue in vivo. RESULTS: Seventeen patients with 24 polyps (13 adenomatous, 11 non-adenomatous) were studied. The autofluorescence decay from adenomas was faster than that from non-adenomas. The measured decay time provided a means of distinguishing adenomas from non-adenomas with a sensitivity of 85%, a specificity of 91%, a positive predictive value of 92%, and a negative predictive value of 83%. CONCLUSIONS: Time-resolved autofluorescence spectroscopy is a promising optical diagnostic technique for determining polyp types in vivo.
Authors: Daniel U Campos-Delgado; O Gutierrez Navarro; E R Arce-Santana; Alex J Walsh; Melissa C Skala; Javier A Jo Journal: Opt Express Date: 2015-09-07 Impact factor: 3.894
Authors: Daniel U Campos-Delgado; Omar Gutierrez-Navarro; Edgar R Arce-Santana; Melissa C Skala; Alex J Walsh; Javier A Jo Journal: J Biomed Opt Date: 2015-07 Impact factor: 3.170
Authors: João L Lagarto; Jennifer E Phipps; Leta Faller; Dinglong Ma; Jakob Unger; Julien Bec; Stephen Griffey; Jonathan Sorger; D Gregory Farwell; Laura Marcu Journal: J Photochem Photobiol B Date: 2018-05-26 Impact factor: 6.252
Authors: Kevin Staniszewski; Said H Audi; Reyhaneh Sepehr; Elizabeth R Jacobs; Mahsa Ranji Journal: Ann Biomed Eng Date: 2012-12-13 Impact factor: 3.934
Authors: Seung Yup Lee; William R Lloyd; Malavika Chandra; Robert H Wilson; Barbara McKenna; Diane Simeone; James Scheiman; Mary-Ann Mycek Journal: Biomed Opt Express Date: 2013-11-14 Impact factor: 3.732
Authors: William R Lloyd; Robert H Wilson; Seung Yup Lee; Malavika Chandra; Barbara McKenna; Diane Simeone; James Scheiman; Mary-Ann Mycek Journal: Biomed Opt Express Date: 2013-12-02 Impact factor: 3.732