Literature DB >> 9776768

Urea improves efficiency of bisulphite-mediated sequencing of 5'-methylcytosine in genomic DNA.

R Paulin1, G W Grigg, M W Davey, A A Piper.   

Abstract

The detection of 5'-methylcytosine by the bisulphite-mediated genomic sequencing method has considerably aided study of the role of methylation in areas such as X chromosome inactivation, genomic imprinting and cancer research. However on occasion difficulty has been experienced in obtaining complete conversion of cytosine to uracil in regions of the target DNA. We report here a simple improvement to the method involving addition of urea to the bisulphite reaction, a step which greatly improves the reaction efficiency, presumably by maintaining the target DNA in single stranded form, thereby allowing complete and reliable conversion.

Entities:  

Mesh：

Substances：

Year:  1998        PMID： 9776768      PMCID： PMC147923          DOI： 10.1093/nar/26.21.5009

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  42 in total

1.  Domain regulation of imprinting cluster in Kip2/Lit1 subdomain on mouse chromosome 7F4/F5: large-scale DNA methylation analysis reveals that DMR-Lit1 is a putative imprinting control region.

Authors:  Hitomi Yatsuki; Keiichiro Joh; Ken Higashimoto; Hidenobu Soejima; Yuji Arai; Youdong Wang; Izuho Hatada; Yayoi Obata; Hiroko Morisaki; Zhongming Zhang; Tetsuji Nakagawachi; Yuji Satoh; Tsunehiro Mukai
Journal:  Genome Res       Date:  2002-12       Impact factor: 9.043

2.  Bisulfite genomic sequencing: systematic investigation of critical experimental parameters.

Authors:  C Grunau; S J Clark; A Rosenthal
Journal:  Nucleic Acids Res       Date:  2001-07-01       Impact factor: 16.971

3.  Low glutelin content1: a dominant mutation that suppresses the glutelin multigene family via RNA silencing in rice.

Authors:  Makoto Kusaba; Kenzo Miyahara; Shuichi Iida; Hiroyuki Fukuoka; Toshiya Takano; Hidenori Sassa; Minoru Nishimura; Takeshi Nishio
Journal:  Plant Cell       Date:  2003-06       Impact factor: 11.277

4.  The small chromosomes of Trypanosoma brucei involved in antigenic variation are constructed around repetitive palindromes.

Authors:  Bill Wickstead; Klaus Ersfeld; Keith Gull
Journal:  Genome Res       Date:  2004-06       Impact factor: 9.043

5.  Analysis of target sequences of DDM1s in Brassica rapa by MSAP.

Authors:  Taku Sasaki; Ryo Fujimoto; Sachie Kishitani; Takeshi Nishio
Journal:  Plant Cell Rep       Date:  2010-11-12       Impact factor: 4.570

6.  Telomerase- and recombination-independent immortalization of budding yeast.

Authors:  Laura Maringele; David Lydall
Journal:  Genes Dev       Date:  2004-10-15       Impact factor: 11.361

7.  Intrastrand annealing leads to the formation of a large DNA palindrome and determines the boundaries of genomic amplification in human cancer.

Authors:  Hisashi Tanaka; Yi Cao; Donald A Bergstrom; Charles Kooperberg; Stephen J Tapscott; Meng-Chao Yao
Journal:  Mol Cell Biol       Date:  2007-01-22       Impact factor: 4.272

8.  CpG hypomethylation in a large domain encompassing the embryonic beta-like globin genes in primitive erythrocytes.

Authors:  Mei Hsu; Rodwell Mabaera; Christopher H Lowrey; David I K Martin; Steven Fiering
Journal:  Mol Cell Biol       Date:  2007-04-23       Impact factor: 4.272

9.  p53 chromatin epigenetic domain organization and p53 transcription.

Authors:  Chia-Hsin Su; Yih-Jyh Shann; Ming-Ta Hsu
Journal:  Mol Cell Biol       Date:  2008-10-20       Impact factor: 4.272

10.  Promiscuous methylation of non-canonical DNA sites by HaeIII methyltransferase.

Authors:  Helen M Cohen; Dan S Tawfik; Andrew D Griffiths
Journal:  Nucleic Acids Res       Date:  2002-09-01       Impact factor: 16.971
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.