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Literature DB >> 9774350

The Escherichia coli OxyS regulatory RNA represses fhlA translation by blocking ribosome binding.

S Altuvia¹, A Zhang, L Argaman, A Tiwari, G Storz.

Abstract

OxyS is a small untranslated RNA which is induced in response to oxidative stress in Escherichia coli. This novel RNA acts as a global regulator to activate or repress the expression of as many as 40 genes, including the fhlA-encoded transcriptional activator and the rpoS-encoded sigma(s) subunit of RNA polymerase. Deletion analysis of OxyS showed that different domains of the small RNA are required for the regulation of fhlA and rpoS. We examined the mechanism of OxyS repression of fhlA and found that the OxyS RNA inhibits fhlA translation by pairing with a short sequence overlapping the Shine-Dalgarno sequence, thereby blocking ribosome binding/translation.

Entities: Gene Species

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Year: 1998 PMID： 9774350 PMCID： PMC1170933 DOI： 10.1093/emboj/17.20.6069

Source DB: PubMed Journal: EMBO J ISSN： 0261-4189 Impact factor: 11.598

23 in total

1. Control of ColE1 plasmid replication. Interaction of Rom protein with an unstable complex formed by RNA I and RNA II.

Authors: J Tomizawa
Journal: J Mol Biol Date: 1990-04-20 Impact factor: 5.469

2. Identification and sequence analysis of the gene encoding the transcriptional activator of the formate hydrogenlyase system of Escherichia coli.

Authors: V Schlensog; A Böck
Journal: Mol Microbiol Date: 1990-08 Impact factor: 3.501

3. Alternative mRNA structures of the cIII gene of bacteriophage lambda determine the rate of its translation initiation.

Authors: S Altuvia; D Kornitzer; D Teff; A B Oppenheim
Journal: J Mol Biol Date: 1989-11-20 Impact factor: 5.469

4. Genetic regulation of formate hydrogenlyase of Escherichia coli: role of the fhlA gene product as a transcriptional activator for a new regulatory gene, fhlB.

Authors: J A Maupin; K T Shanmugam
Journal: J Bacteriol Date: 1990-09 Impact factor: 3.490

5. Improved single and multicopy lac-based cloning vectors for protein and operon fusions.

Authors: R W Simons; F Houman; N Kleckner
Journal: Gene Date: 1987 Impact factor: 3.688

6. The function of micF RNA. micF RNA is a major factor in the thermal regulation of OmpF protein in Escherichia coli.

Authors: J Andersen; S A Forst; K Zhao; M Inouye; N Delihas
Journal: J Biol Chem Date: 1989-10-25 Impact factor: 5.157

7. Mutagenesis of plasmid DNA with hydroxylamine: isolation of mutants of multi-copy plasmids.

Authors: G O Humphreys; G A Willshaw; H R Smith; E S Anderson
Journal: Mol Gen Genet Date: 1976-04-23

8. micF RNA binds to the 5' end of ompF mRNA and to a protein from Escherichia coli.

Authors: J Andersen; N Delihas
Journal: Biochemistry Date: 1990-10-02 Impact factor: 3.162

9. Genetic analysis of bacteriophage lambda cIII gene: mRNA structural requirements for translation initiation.

Authors: D Kornitzer; D Teff; S Altuvia; A B Oppenheim
Journal: J Bacteriol Date: 1989-05 Impact factor: 3.490

10. The IS10 antisense RNA blocks ribosome binding at the transposase translation initiation site.

Authors: C Ma; R W Simons
Journal: EMBO J Date: 1990-04 Impact factor: 11.598

76 in total

Review 1. Switching on and off with RNA.

Authors: S Altuvia; E G Wagner
Journal: Proc Natl Acad Sci U S A Date: 2000-08-29 Impact factor: 11.205

Review 2. Signal transduction and regulatory mechanisms involved in control of the sigma(S) (RpoS) subunit of RNA polymerase.

Authors: Regine Hengge-Aronis
Journal: Microbiol Mol Biol Rev Date: 2002-09 Impact factor: 11.056

The Escherichia coli OxyS regulatory RNA represses fhlA translation by blocking ribosome binding.

1. Control of ColE1 plasmid replication. Interaction of Rom protein with an unstable complex formed by RNA I and RNA II.

2. Identification and sequence analysis of the gene encoding the transcriptional activator of the formate hydrogenlyase system of Escherichia coli.

3. Alternative mRNA structures of the cIII gene of bacteriophage lambda determine the rate of its translation initiation.

4. Genetic regulation of formate hydrogenlyase of Escherichia coli: role of the fhlA gene product as a transcriptional activator for a new regulatory gene, fhlB.

5. Improved single and multicopy lac-based cloning vectors for protein and operon fusions.

6. The function of micF RNA. micF RNA is a major factor in the thermal regulation of OmpF protein in Escherichia coli.

7. Mutagenesis of plasmid DNA with hydroxylamine: isolation of mutants of multi-copy plasmids.

8. micF RNA binds to the 5' end of ompF mRNA and to a protein from Escherichia coli.

9. Genetic analysis of bacteriophage lambda cIII gene: mRNA structural requirements for translation initiation.

10. The IS10 antisense RNA blocks ribosome binding at the transposase translation initiation site.

Review 1. Switching on and off with RNA.

Review 2. Signal transduction and regulatory mechanisms involved in control of the sigma(S) (RpoS) subunit of RNA polymerase.

3. Predicted structure and phyletic distribution of the RNA-binding protein Hfq.

Review 4. Structure and functional properties of prokaryotic small noncoding RNAs.

5. Regulatory small RNAs: the key to coordinating global regulatory circuits.

6. sRNATarBase: a comprehensive database of bacterial sRNA targets verified by experiments.

7. Slowly produced microRNAs control protein levels.

8. Disruption of small RNA signaling caused by competition for Hfq.

Review 9. Bacterial small RNA regulators: versatile roles and rapidly evolving variations.

10. The small RNA GcvB regulates sstT mRNA expression in Escherichia coli.