Literature DB >> 9765587

The nine genes of the Nocardia lactamdurans cephamycin cluster are transcribed into large mRNAs from three promoters, two of them located in a bidirectional promoter region.

F J Enguita1, J J Coque, P Liras, J F Martin.   

Abstract

The nine biosynthesis genes of the Nocardia lactamdurans cephamycin cluster are expressed as three different mRNAs initiating at promoters latp, cefDp, and pcbABp, as shown by low-resolution S1 nuclease protection assays and Northern blotting analysis. Bidirectional expression occurred from divergent promoters (latp and cefDp) located in a 629-bp intergenic region that contains three heptameric direct repeats similar to those recognized by members of the SARP (Streptomyces antibiotic regulatory proteins) family. The lat gene is transcribed in a single monocistronic transcript initiating at latp. A second unusually long polycistronic mRNA (more than 16 kb) corresponding to six biosynthesis genes (pcbAB, pcbC, cmcI, cmcJ, cefF, and cmcH) started at pcbABp. A third polycistronic mRNA corresponding to the cefD and cefE genes started at cefDp.

Entities:  

Mesh：

Substances：

Year:  1998        PMID： 9765587      PMCID： PMC107604     

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  22 in total

1.  A novel family of proteins that regulates antibiotic production in streptomycetes appears to contain an OmpR-like DNA-binding fold.

Authors:  A Wietzorrek; M Bibb
Journal:  Mol Microbiol       Date:  1997-09       Impact factor: 3.501

Review 2.  Divergent promoters, a common form of gene organization.

Authors:  C F Beck; R A Warren
Journal:  Microbiol Rev       Date:  1988-09

3.  Nucleotide sequences encoding and promoting expression of three antibiotic resistance genes indigenous to Streptomyces.

Authors:  M J Bibb; M J Bibb; J M Ward; S N Cohen
Journal:  Mol Gen Genet       Date:  1985

4.  Streptomyces promoter-probe plasmids that utilise the xylE gene of Pseudomonas putida.

Authors:  T M Clayton; M J Bibb
Journal:  Nucleic Acids Res       Date:  1990-02-25       Impact factor: 16.971

Review 5.  Organization and expression of genes involved in the biosynthesis of antibiotics and other secondary metabolites.

Authors:  M F Martín; P Liras
Journal:  Annu Rev Microbiol       Date:  1989       Impact factor: 15.500

6.  The beta-lactam biosynthesis genes for isopenicillin N epimerase and deacetoxycephalosporin C synthetase are expressed from a single transcript in Streptomyces clavuligerus.

Authors:  S Kovacevic; M B Tobin; J R Miller
Journal:  J Bacteriol       Date:  1990-07       Impact factor: 3.490

7.  xylE functions as an efficient reporter gene in Streptomyces spp.: use for the study of galP1, a catabolite-controlled promoter.

Authors:  C Ingram; M Brawner; P Youngman; J Westpheling
Journal:  J Bacteriol       Date:  1989-12       Impact factor: 3.490

8.  The cephamycin biosynthetic genes pcbAB, encoding a large multidomain peptide synthetase, and pcbC of Nocardia lactamdurans are clustered together in an organization different from the same genes in Acremonium chrysogenum and Penicillium chrysogenum.

Authors:  J J Coque; J F Martín; J G Calzada; P Liras
Journal:  Mol Microbiol       Date:  1991-05       Impact factor: 3.501

9.  Relationship between nitrogen assimilation and cephalosporin synthesis in Streptomyces clavuligerus.

Authors:  A F Brana; S Wolfe; A L Demain
Journal:  Arch Microbiol       Date:  1986-10       Impact factor: 2.552

10.  A gene encoding lysine 6-aminotransferase, which forms the beta-lactam precursor alpha-aminoadipic acid, is located in the cluster of cephamycin biosynthetic genes in Nocardia lactamdurans.

Authors:  J J Coque; P Liras; L Laiz; J F Martín
Journal:  J Bacteriol       Date:  1991-10       Impact factor: 3.490
View more
  4 in total

1.  Early cephamycin biosynthetic genes are expressed from a polycistronic transcript in Streptomyces clavuligerus.

Authors:  D C Alexander; M J Brumlik; L Lee; S E Jensen
Journal:  J Bacteriol       Date:  2000-01       Impact factor: 3.490

2.  Inducing effect of diamines on transcription of the cephamycin C genes from the lat and pcbAB promoters in Nocardia lactamdurans.

Authors:  A L Leitão; F J Enguita; J L De La Fuente; P Liras; J F Martin
Journal:  J Bacteriol       Date:  1999-04       Impact factor: 3.490

3.  Transcriptional organization and regulation of magnetosome operons in Magnetospirillum gryphiswaldense.

Authors:  Sabrina Schübbe; Chris Würdemann; Jörg Peplies; Udo Heyen; Cathrin Wawer; Frank Oliver Glöckner; Dirk Schüler
Journal:  Appl Environ Microbiol       Date:  2006-09       Impact factor: 4.792

Review 4.  Roles of 2-oxoglutarate oxygenases and isopenicillin N synthase in β-lactam biosynthesis.

Authors:  Patrick Rabe; Jos J A G Kamps; Christopher J Schofield; Christopher T Lohans
Journal:  Nat Prod Rep       Date:  2018-08-15       Impact factor: 13.423
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.