Literature DB >> 9762137

Quantitative Limulus lysate assay for endotoxin activity: aggregation of radioiodinated coagulogen monomers.

R S Munford1.   

Abstract

This communication describes a modification of the Limulus lysate assay which allows precise quantitation of picograms of bacterial lipopolysaccharide activity. The method measures the incorporation of 125I-labeled coagulogen monomers into the lysate clot as a function of lipopolysaccharide concentration. The method is more precise and requires less lysate than the previously described quantitative assays for endotoxin activity.

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Year:  1978        PMID: 9762137     DOI: 10.1016/0003-2697(78)90537-7

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


  11 in total

1.  Radioimmunoassay for Gram-negative bacterial lipopolysaccharide O antigens: influence of antigen solubility.

Authors:  R S Munford; C L Hall
Journal:  Infect Immun       Date:  1979-10       Impact factor: 3.441

2.  Detection of free endotoxin in cerebrospinal fluid by the Limulus lysate test.

Authors:  R S Munford; C L Hall; L Grimm
Journal:  Infect Immun       Date:  1984-08       Impact factor: 3.441

3.  Endotoxin testing revisited.

Authors:  A Sturk; J W ten Cate
Journal:  Eur J Clin Microbiol       Date:  1985-08       Impact factor: 3.267

4.  Modified micromethod of the Limulus amoebocyte lysate assay for endotoxin.

Authors:  K L Melvaer; D Fystro
Journal:  Appl Environ Microbiol       Date:  1982-02       Impact factor: 4.792

5.  Automation of the Limulus amoebocyte lysate test by using the Abbott MS-2 microbiology system.

Authors:  J H Jorgensen; G A Alexander
Journal:  Appl Environ Microbiol       Date:  1981-06       Impact factor: 4.792

6.  Adaptation of a microdilution procedure to the Limulus lysate assay for endotoxin.

Authors:  R B Prior; V A Spagna
Journal:  J Clin Microbiol       Date:  1979-09       Impact factor: 5.948

7.  Local immune response to Escherichia coli pili in experimental pyelonephritis.

Authors:  J W Smith; S Wagner; R M Swenson
Journal:  Infect Immun       Date:  1981-01       Impact factor: 3.441

8.  Comparative evaluation of the tube and microdilution Limulus lysate techniques for rapid presumptive diagnosis of gonococcal urethritis in men.

Authors:  R B Prior; V A Spagna
Journal:  J Clin Microbiol       Date:  1980-04       Impact factor: 5.948

9.  Biological activity, lipoprotein-binding behavior, and in vivo disposition of extracted and native forms of Salmonella typhimurium lipopolysaccharides.

Authors:  R S Munford; C L Hall; J M Lipton; J M Dietschy
Journal:  J Clin Invest       Date:  1982-10       Impact factor: 14.808

10.  New, sensitive rocket immunoelectrophoretic assay for measurement of the reaction between endotoxin and Limulus amoebocyte lysate.

Authors:  L Baek
Journal:  J Clin Microbiol       Date:  1983-06       Impact factor: 5.948

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