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Literature DB >> 9755589

Immunodiagnosis of clonorchiasis using a recombinant antigen.

T S Yong¹, H J Yang, S J Park, Y K Kim, D H Lee, S M Lee.

Abstract

A cDNA expression library of Clonorchis sinensis adult worm was constructed, and screened out immunologically. One clone, pBCs31, was selected in view of its predominant reactivity with an experimentally infected rabbit serum. Recombinant C. sinensis antigen with 28 kDa as a beta-galactosidase fusion protein produced in Escherichia coli was identified by immunoblot analysis. The cloned gene was composed of 16 copies of a 30 base pair repeat and an additional 320 bases. The deduced amino acid sequence of the tandem repeat was AQPPKSGDGG. On RNA slot blot analysis. C. sinensis adult worm RNA showed a positive reaction with the cloned gene. Enzyme-linked immunosorbent assay using a purified recombinant antigen of pBCs31 showed high specificity for diagnosis of clonorchiasis.

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Year: 1998 PMID： 9755589 PMCID： PMC2732929 DOI： 10.3347/kjp.1998.36.3.183

Source DB: PubMed Journal: Korean J Parasitol ISSN： 0023-4001 Impact factor: 1.341

13 in total

1. The current pathobiology and chemotherapy of clonorchiasis.

Authors: Han Jong Rim
Journal: Kisaengchunghak Chapchi Date: 1986-04

2. Cloning of a major surface-antigen gene of Trypanosoma cruzi and identification of a nonapeptide repeat.

Authors: D S Peterson; R A Wrightsman; J E Manning
Journal: Nature Date: 1986 Aug 7-13 Impact factor: 49.962

3. Enzyme-linked immunoelectrotransfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis.

Authors: V C Tsang; J M Peralta; A R Simons
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4. Immunoblot patterns of clonorchiasis.

Authors: S T Hong; W G Kho; M Lee; J S Lee; S H Lee
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5. A Clonorchis sinensis-specific antigen that detects active human clonorchiasis.

Authors: S I Kim
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6. Analysis of Clonorchis sinensis antigens and diagnosis of clonorchiasis using monoclonal antibodies.

Authors: T S Yong; K Im; P R Chung
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7. Dermal glands of Xenopus laevis contain a polypeptide with a highly repetitive amino acid sequence.

Authors: M Gmachl; H Berger; J Thalhammer; G Kreil
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8. Detection of specific serum IgE in clonorchiasis cases and analysis of Clonorchis sinensis allergens.

Authors: T S Yong; D S Kim; S Y Lee; K I Im; K Y Lee
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9. Clonorchis sinensis tropomyosin: cloning and sequence of partial cDNA amplified by PCR.

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10. Structure of the gene encoding the immunodominant surface antigen on the sporozoite of the human malaria parasite Plasmodium falciparum.

Authors: J B Dame; J L Williams; T F McCutchan; J L Weber; R A Wirtz; W T Hockmeyer; W L Maloy; J D Haynes; I Schneider; D Roberts
Journal: Science Date: 1984-08-10 Impact factor: 47.728

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7. Antigenic profile and localization of Clonorchis sinensis proteins in the course of infection.

Authors: S J Hong; T Y Kim; K Y Song; W M Sohn; S Y Kang
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9. Characterization of partially purified 8 kDa antigenic protein of Clonorchis sinensis.

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10. Development of a polymerase chain reaction applicable to rapid and sensitive detection of Clonorchis sinensis eggs in human stool samples.

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