Literature DB >> 9746536

The active site structure of tetanus neurotoxin resolved by multiple scattering analysis in X-Ray absorption spectroscopy.

C Meneghini1, S Morante.   

Abstract

A detailed study of the x-ray absorption spectrum of tetanus neurotoxin in the K-edge EXAFS region of the zinc absorber is presented that allows the complete identification of the amino acid residues coordinated to the zinc active site. A very satisfactory interpretation of the experimental data can be given if multiple scattering contributions are included in the analysis. Comparing the absorption spectrum of tetanus neurotoxin to that of two other structurally similar zinc-endopeptidases, thermolysin and astacin, in which the zinc coordination mode is known from crystallographic data, we conclude that in tetanus neurotoxin, besides a water molecule, zinc is coordinated to two histidines and a tyrosine.

Entities:  

Mesh：

Substances：

Year:  1998        PMID： 9746536      PMCID： PMC1299866          DOI： 10.1016/S0006-3495(98)77636-2

Source DB:  PubMed          Journal:  Biophys J        ISSN： 0006-3495            Impact factor:   4.033


  21 in total

1.  Multiple-scattering regime and higher-order correlations in x-ray-absorption spectra of liquid solutions.

Authors: 
Journal:  Phys Rev B Condens Matter       Date:  1986-10-15

2.  X-ray-absorption spectroscopy and n-body distribution functions in condensed matter. II. Data analysis and applications.

Authors: 
Journal:  Phys Rev B Condens Matter       Date:  1995-12-01

3.  Secondary structural predictions for the clostridial neurotoxins.

Authors:  F J Lebeda; M A Olson
Journal:  Proteins       Date:  1994-12

4.  An extended X-ray-absorption-fine-structure study of the copper and zinc sites of freeze-dried bovine superoxide dismutase.

Authors:  N J Blackburn; S S Hasnain; G P Diakun; P F Knowles; N Binsted; C D Garner
Journal:  Biochem J       Date:  1983-09-01       Impact factor: 3.857

5.  SNARE motif and neurotoxins.

Authors:  O Rossetto; G Schiavo; C Montecucco; B Poulain; F Deloye; L Lozzi; C C Shone
Journal:  Nature       Date:  1994-12-01       Impact factor: 49.962

6.  Three-dimensional structure of the alkaline protease of Pseudomonas aeruginosa: a two-domain protein with a calcium binding parallel beta roll motif.

Authors:  U Baumann; S Wu; K M Flaherty; D B McKay
Journal:  EMBO J       Date:  1993-09       Impact factor: 11.598

7.  The structure of calsequestrin in triads of vertebrate skeletal muscle: a deep-etch study.

Authors:  C Franzini-Armstrong; L J Kenney; E Varriano-Marston
Journal:  J Cell Biol       Date:  1987-07       Impact factor: 10.539

8.  Botulinum neurotoxins are zinc proteins.

Authors:  G Schiavo; O Rossetto; A Santucci; B R DasGupta; C Montecucco
Journal:  J Biol Chem       Date:  1992-11-25       Impact factor: 5.157

Review 9.  Mechanism of action of tetanus and botulinum neurotoxins.

Authors:  C Montecucco; G Schiavo
Journal:  Mol Microbiol       Date:  1994-07       Impact factor: 3.501

Review 10.  Tetanus and botulism neurotoxins: a new group of zinc proteases.

Authors:  C Montecucco; G Schiavo
Journal:  Trends Biochem Sci       Date:  1993-09       Impact factor: 13.807
View more
  10 in total

1.  An x-ray absorption spectroscopy study of the zinc environment in Langmuir-Blodgett phospholipid multilayers.

Authors:  S Nuzzo; C Meneghini; S Mobilioo; H Haas; P Riccio; A Fasano; P Cavatorta; S Morante
Journal:  Biophys J       Date:  2002-12       Impact factor: 4.033

2.  A new method to determine the structure of the metal environment in metalloproteins: investigation of the prion protein octapeptide repeat Cu(2+) complex.

Authors:  Matthias Mentler; Andreas Weiss; Klaus Grantner; Pablo del Pino; Dominga Deluca; Stella Fiori; Christian Renner; Wolfram Meyer Klaucke; Luis Moroder; Uwe Bertsch; Hans A Kretzschmar; Paul Tavan; Fritz G Parak
Journal:  Eur Biophys J       Date:  2004-09-28       Impact factor: 1.733

3.  Multiple scattering x-ray absorption studies of Zn2+ binding sites in bacterial photosynthetic reaction centers.

Authors:  Lisa Giachini; Francesco Francia; Antonia Mallardi; Gerardo Palazzo; Emilio Carpenè; Federico Boscherini; Giovanni Venturoli
Journal:  Biophys J       Date:  2004-12-21       Impact factor: 4.033

4.  Identifying the structure of the active sites of human recombinant prolidase.

Authors:  Roberta Besio; Stefania Alleva; Antonella Forlino; Anna Lupi; Carlo Meneghini; Velia Minicozzi; Antonella Profumo; Francesco Stellato; Ruggero Tenni; Silvia Morante
Journal:  Eur Biophys J       Date:  2009-05-05       Impact factor: 1.733

5.  pH-dependent local structure of ferricytochrome c studied by x-ray absorption spectroscopy.

Authors:  F Boffi; A Bonincontro; S Cinelli; A Congiu Castellano; A De Francesco; S Della Longa; M Girasole; G Onori
Journal:  Biophys J       Date:  2001-03       Impact factor: 4.033

6.  Metal binding in amyloid beta-peptides shows intra- and inter-peptide coordination modes.

Authors:  Francesco Stellato; Gianfranco Menestrina; Mauro Dalla Serra; Cristina Potrich; Rossella Tomazzolli; Wolfram Meyer-Klaucke; Silvia Morante
Journal:  Eur Biophys J       Date:  2006-01-11       Impact factor: 1.733

7.  Oxidized derivatives of Octopus vulgaris and Carcinus aestuarii hemocyanins at pH 7.5 and related models by x-ray absorption spectroscopy.

Authors:  Elena Borghi; Pier Lorenzo Solari; Mariano Beltramini; Luigi Bubacco; Paolo Di Muro; Benedetto Salvato
Journal:  Biophys J       Date:  2002-06       Impact factor: 4.033

8.  Aggregates Sealed by Ions.

Authors:  Giovanni La Penna; Silvia Morante
Journal:  Methods Mol Biol       Date:  2022

9.  An XAS study of the sulfur environment in human neuromelanin and its synthetic analogs.

Authors:  Pier Raimondo Crippa; Melvin Eisner; Silvia Morante; Francesco Stellato; Flavio C Vicentin; Luigi Zecca
Journal:  Eur Biophys J       Date:  2009-05-27       Impact factor: 1.733

10.  The five-to-six-coordination transition of ferric human serum heme-albumin is allosterically-modulated by ibuprofen and warfarin: a combined XAS and MD study.

Authors:  Carlo Meneghini; Loris Leboffe; Monica Bionducci; Gabriella Fanali; Massimiliano Meli; Giorgio Colombo; Mauro Fasano; Paolo Ascenzi; Settimio Mobilio
Journal:  PLoS One       Date:  2014-08-25       Impact factor: 3.240
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.