M Kantorow1, J Horwitz, D Carper. 1. Jules Stein Eye Institute, Los Angeles, CA 90095-7065, USA. kantorow@jseint.jsei.ucla.edu
Abstract
PURPOSE: To characterize gene expression patterns between epithelia isolated from cataractous and normal human lenses. METHODS: Reverse transcriptase differential display was used to identify differential expression between cataractous and normal epithelia. RT-PCR was used to compare pooled and individual RNA samples. RESULTS: One transcript, up-regulated in cataractous as compared to normal epithelia, was identified as osteonectin which is also known as SPARC (secreted acidic protein rich in cysteines). RT-PCR confirmed over-expression of this RNA. High levels of osteonectin mRNA were also detected in six individual epithelia dissected from cataractous lenses. CONCLUSIONS: The present study provides evidence for up-regulation of osteonectin in human age-related cataract and suggests that osteonectin, a protein involved in cell-cycle control, extracellular matrix and Ca++ binding, plays an important role in human lens homeostasis and may be involved in processes leading to lens opacity.
PURPOSE: To characterize gene expression patterns between epithelia isolated from cataractous and normal human lenses. METHODS: Reverse transcriptase differential display was used to identify differential expression between cataractous and normal epithelia. RT-PCR was used to compare pooled and individual RNA samples. RESULTS: One transcript, up-regulated in cataractous as compared to normal epithelia, was identified as osteonectin which is also known as SPARC (secreted acidic protein rich in cysteines). RT-PCR confirmed over-expression of this RNA. High levels of osteonectin mRNA were also detected in six individual epithelia dissected from cataractous lenses. CONCLUSIONS: The present study provides evidence for up-regulation of osteonectin in human age-related cataract and suggests that osteonectin, a protein involved in cell-cycle control, extracellular matrix and Ca++ binding, plays an important role in humanlens homeostasis and may be involved in processes leading to lens opacity.
Authors: WeiYan Zhang; John Hawse; QingLing Huang; Nancy Sheets; Kevin M Miller; Joseph Horwitz; Marc Kantorow Journal: Invest Ophthalmol Vis Sci Date: 2002-01 Impact factor: 4.799
Authors: John R Hawse; James F Hejtmancik; Quingling Huang; Nancy L Sheets; Douglas A Hosack; Richard A Lempicki; Joseph Horwitz; Marc Kantorow Journal: Mol Vis Date: 2003-10-07 Impact factor: 2.367