Literature DB >> 9740721

Reconstitution of chromatin complexes from high-performance liquid chromatography-purified histones.

J Ausió1, S C Moore.   

Abstract

We describe a method to reconstitute chromatin complexes from reversed-phase high-performance liquid chromatography (HPLC)-purified histones. The complexes reconstituted in this way exhibit the same structural characteristics as their equivalent native counterparts. Furthermore, this method works independently of the acid- or salt-extracted origin of the histones used for the HPLC fractionation. The potential of this method for the reconstitution of chromatin particles consisting of sequence-defined DNA templates and well-defined histone variants and/or their posttranslationally modified isoforms is discussed.

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Year:  1998        PMID： 9740721     DOI： 10.1006/meth.1998.0637

Source DB:  PubMed          Journal:  Methods        ISSN： 1046-2023            Impact factor:   3.608


  12 in total

1.  MBD4-mediated glycosylase activity on a chromatin template is enhanced by acetylation.

Authors:  Toyotaka Ishibashi; Kevin So; Claire G Cupples; Juan Ausió
Journal:  Mol Cell Biol       Date:  2008-06-02       Impact factor: 4.272

2.  The characterization of macroH2A beyond vertebrates supports an ancestral origin and conserved role for histone variants in chromatin.

Authors:  Ciro Rivera-Casas; Rodrigo Gonzalez-Romero; Manjinder S Cheema; Juan Ausió; José M Eirín-López
Journal:  Epigenetics       Date:  2016-04-15       Impact factor: 4.528

3.  Phosphorylation of histone H2A.X by DNA-dependent protein kinase is not affected by core histone acetylation, but it alters nucleosome stability and histone H1 binding.

Authors:  Andra Li; Yaping Yu; Sheng-Chun Lee; Toyotaka Ishibashi; Susan P Lees-Miller; Juan Ausió
Journal:  J Biol Chem       Date:  2010-03-31       Impact factor: 5.157

4.  Loss of a DNA binding site within the tail of prelamin A contributes to altered heterochromatin anchorage by progerin.

Authors:  Francine Bruston; Erwan Delbarre; Cecilia Ostlund; Howard J Worman; Brigitte Buendia; Isabelle Duband-Goulet
Journal:  FEBS Lett       Date:  2010-05-24       Impact factor: 4.124

Review 5.  Chemical approaches to understand the language of histone modifications.

Authors:  Abhinav Dhall; Champak Chatterjee
Journal:  ACS Chem Biol       Date:  2011-08-17       Impact factor: 5.100

6.  Acetylation of vertebrate H2A.Z and its effect on the structure of the nucleosome.

Authors:  Toyotaka Ishibashi; Deanna Dryhurst; Kristie L Rose; Jeffrey Shabanowitz; Donald F Hunt; Juan Ausió
Journal:  Biochemistry       Date:  2009-06-09       Impact factor: 3.162

7.  Histone H2A (H2A.X and H2A.Z) variants in molluscs: molecular characterization and potential implications for chromatin dynamics.

Authors:  Rodrigo González-Romero; Ciro Rivera-Casas; Lindsay J Frehlick; Josefina Méndez; Juan Ausió; José M Eirín-López
Journal:  PLoS One       Date:  2012-01-11       Impact factor: 3.240

8.  Interaction of chromatin with a histone H1 containing swapped N- and C-terminal domains.

Authors:  Jordana B Hutchinson; Manjinder S Cheema; Jason Wang; Krystal Missiaen; Ron Finn; Rodrigo Gonzalez Romero; John P H Th'ng; Michael Hendzel; Juan Ausió
Journal:  Biosci Rep       Date:  2015-04-27       Impact factor: 3.840

9.  Expression and purification of the full murine NPM2 and study of its interaction with protamines and histones.

Authors:  Katherine Ellard; Jason J Serpa; Evgeniy V Petrotchenko; Christoph H Borchers; Juan Ausió
Journal:  Biochem Biophys Rep       Date:  2016-04-08

Review 10.  The Structural Determinants behind the Epigenetic Role of Histone Variants.

Authors:  Manjinder S Cheema; Juan Ausió
Journal:  Genes (Basel)       Date:  2015-07-23       Impact factor: 4.096
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