Literature DB >> 972853

Separation of Met-tRNAf and Met-tRNAm by chromatography on Sepharose 4B columns.

O Nygård, T Hultin.   

Abstract

Unfractionated rabbit liver tRNA, charged with [3H]methionine by use of rat liver enzymes, was separated into two [3H5methionine-containing fractions by column chromatography on Sepharose 4B. The two fractions were identified as Met-tRNAMetm and Met-tRNAMetf by (a) their different ability for form a GTP-dependent ternary complex with IF-MP, and (b) the absence of the first fraction after selective charging of the tRNA with E. coli amino acyl tRNA synthetase. The methionine residue was without noticeable influence on the separation.

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Year:  1976        PMID： 972853     DOI： 10.1080/00327487608061623

Source DB:  PubMed          Journal:  Prep Biochem        ISSN： 0032-7484


  4 in total

1.  Cross-linking of mRNA to initiation factor eIF-3, 24 kDa cap binding protein and ribosomal proteins S1, S3/3a, S6 and S11 within the 48S pre-initiation complex.

Authors:  P Westermann; O Nygård
Journal:  Nucleic Acids Res       Date:  1984-12-11       Impact factor: 16.971

2.  The alpha and gamma subunits of initiation factor eIF-2 can be cross-linked to 18S ribosomal RNA within the quaternary initiation complex, eIF-2.Met-tRNAf.GDPCP.small ribosomal subunit.

Authors:  P Westermann; O Nygård; H Bielka
Journal:  Nucleic Acids Res       Date:  1980-07-25       Impact factor: 16.971

3.  Cross-linking of Met-tRNAf to eIF-2 beta and to the ribosomal proteins S3a and S6 within the eukaryotic inhibition complex, eIF-2 .GMPPCP.Met-tRNAf.small ribosomal subunit.

Authors:  P Westermann; O Nygård; H Bielka
Journal:  Nucleic Acids Res       Date:  1981-05-25       Impact factor: 16.971

4.  Early effects of dimethylnitrosamine on the initiation of protein synthesis in mouse liver.

Authors:  O Nygård; T Hultin
Journal:  Biochem J       Date:  1981-02-15       Impact factor: 3.857
  4 in total

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