Literature DB >> 9722654

A rapid and sensitive method to measure the enzymatic activity of ribosome-inactivating proteins.

M Brigotti1, L Barbieri, P Valbonesi, F Stirpe, L Montanaro, S Sperti.   

Abstract

A method is described in which the adenosine- N -glycosidase activity of ribosome-inactivating proteins (RIPs) is measured using as substrate a 2251 bp [3H]DNA obtained by PCR amplification of the 731-2981 region of the pBR322 plasmid. The DNA, labelled in the purine ring of adenine, proved a good substrate for all three RIPs tested (PAP-S, ricin and shiga-like toxin I). The method, which measures directly the [3H]adenine released, is highly specific, extremely rapid and quantitative in a wide range of RIP concentrations.

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Year:  1998        PMID： 9722654      PMCID： PMC147822          DOI： 10.1093/nar/26.18.4306

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  7 in total

1.  Ribosome inactivation by the toxic lectins abrin and ricin. Kinetics of the enzymic activity of the toxin A-chains.

Authors:  S Olsnes; C Fernandez-Puentes; L Carrasco; D Vazquez
Journal:  Eur J Biochem       Date:  1975-12-01

2.  The RNA-N-glycosidase activity of Shiga-like toxin I: kinetic parameters of the native and activated toxin.

Authors:  M Brigotti; D Carnicelli; P Alvergna; R Mazzaracchio; S Sperti; L Montanaro
Journal:  Toxicon       Date:  1997-09       Impact factor: 3.033

3.  Polynucleotide:adenosine glycosidase activity of ribosome-inactivating proteins: effect on DNA, RNA and poly(A).

Authors:  L Barbieri; P Valbonesi; E Bonora; P Gorini; A Bolognesi; F Stirpe
Journal:  Nucleic Acids Res       Date:  1997-02-01       Impact factor: 16.971

Review 4.  Ribosome-inactivating proteins from plants.

Authors:  L Barbieri; M G Battelli; F Stirpe
Journal:  Biochim Biophys Acta       Date:  1993-12-21

5.  High-pressure-liquid-chromatographic and fluorimetric methods for the determination of adenine released from ribosomes by ricin and gelonin.

Authors:  M Zamboni; M Brigotti; F Rambelli; L Montanaro; S Sperti
Journal:  Biochem J       Date:  1989-05-01       Impact factor: 3.857

6.  The RNA N-glycosidase activity of ricin A-chain. The characteristics of the enzymatic activity of ricin A-chain with ribosomes and with rRNA.

Authors:  Y Endo; K Tsurugi
Journal:  J Biol Chem       Date:  1988-06-25       Impact factor: 5.157

7.  The mechanism of action of ricin and related toxic lectins on eukaryotic ribosomes. The site and the characteristics of the modification in 28 S ribosomal RNA caused by the toxins.

Authors:  Y Endo; K Mitsui; M Motizuki; K Tsurugi
Journal:  J Biol Chem       Date:  1987-04-25       Impact factor: 5.157
  7 in total
  7 in total

1.  Identity elements in bovine tRNA(Trp) required for the specific stimulation of gelonin, a plant ribosome-inactivating protein.

Authors:  M Brigotti; D Carnicelli; A Pallanca; S Rizzi; P Accorsi; L Montanaro; S Sperti
Journal:  RNA       Date:  1999-10       Impact factor: 4.942

2.  Change in conformation with reduction of alpha-helix content causes loss of neutrophil binding activity in fully cytotoxic Shiga toxin 1.

Authors:  Maurizio Brigotti; Domenica Carnicelli; Valentina Arfilli; Laura Rocchi; Francesca Ricci; Pasqualepaolo Pagliaro; Pier Luigi Tazzari; Antonio González Vara; Matteo Amelia; Francesco Manoli; Sandra Monti
Journal:  J Biol Chem       Date:  2011-08-08       Impact factor: 5.157

3.  Molecular damage and induction of proinflammatory cytokines in human endothelial cells exposed to Shiga toxin 1, Shiga toxin 2, and alpha-sarcin.

Authors:  Maurizio Brigotti; Domenica Carnicelli; Elisa Ravanelli; Antonio González Vara; Chiara Martinelli; Roberta R Alfieri; Pier Giorgio Petronini; Piero Sestili
Journal:  Infect Immun       Date:  2007-02-12       Impact factor: 3.441

4.  Development of a quantitative RT-PCR assay to examine the kinetics of ribosome depurination by ribosome inactivating proteins using Saccharomyces cerevisiae as a model.

Authors:  Michael Pierce; Jennifer Nielsen Kahn; Jiachi Chiou; Nilgun E Tumer
Journal:  RNA       Date:  2010-11-23       Impact factor: 4.942

5.  4-Aminopyrazolo[3,4-d]pyrimidine (4-APP) as a novel inhibitor of the RNA and DNA depurination induced by Shiga toxin 1.

Authors:  M Brigotti; D Carnicelli; P Accorsi; S Rizzi; L Montanaro; S Sperti
Journal:  Nucleic Acids Res       Date:  2000-06-15       Impact factor: 16.971

6.  Improved Sensitivity for the Qualitative and Quantitative Analysis of Active Ricin by MALDI-TOF Mass Spectrometry.

Authors:  Dongxia Wang; Jakub Baudys; John R Barr; Suzanne R Kalb
Journal:  Anal Chem       Date:  2016-06-15       Impact factor: 6.986

7.  Whole-Cell Multiparameter Assay for Ricin and Abrin Activity-Based Digital Holographic Microscopy.

Authors:  Efi Makdasi; Orly Laskar; Elad Milrot; Ofir Schuster; Shlomo Shmaya; Shmuel Yitzhaki
Journal:  Toxins (Basel)       Date:  2019-03-22       Impact factor: 4.546
  7 in total

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