| Literature DB >> 9721239 |
J Schaefer-Klein1, I Givol, E V Barsov, J M Whitcomb, M VanBrocklin, D N Foster, M J Federspiel, S H Hughes.
Abstract
The lack of a well-behaved permanent, adherent, nontransformed chicken cell line has made some experiments with avian leukosis-sarcoma viruses (ASLV) and vectors considerably more difficult. The EV-O-derived line, DF-1, supports the efficient replication of subgroups (A), (B), and (C) ASLV, as well as amphotrophic murine leukemia virus and an ASLV-derived vector that has its env gene derived from the env gene from an amphotrophic murine leukemia virus. The cell line responds appropriately to the expression of a transforming oncogene (v-myc) to a growth suppressor gene [p21(waf1)] and can be sorted (using FACS) if infected by an ASLV vector that expresses GFP. Copyright 1998 Academic Press.Entities:
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Year: 1998 PMID： 9721239 DOI： 10.1006/viro.1998.9291
Source DB: PubMed Journal: Virology ISSN： 0042-6822 Impact factor: 3.616