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Literature DB >> 9707550

Promoter-proximal stalling results from the inability to recruit transcription factor IIH to the transcription complex and is a regulated event.

Abstract

Promoter-proximal stalling, a general phenomenon observed during the expression of many RNA polymerase II transcribed genes, is dependent on transcription factor IIH (TFIIH). Reactions lacking TFIIH initiated transcription, but the transcription complex encountered a block to elongation proximal to the promoter. The accumulation of promoter-proximal stalled complexes was reduced in the presence of TFIIH and efficient escape from this site also required an activator. Promoter-proximal stalled complexes could not be induced to resume elongation. Our results indicate that effective recruitment of TFIIH into transcription complexes is achieved during formation of the preinitiation complex at the promoter. The studies establish that promoter clearance is a regulated event that requires TFIIH.

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Year: 1998 PMID： 9707550 PMCID： PMC21411 DOI： 10.1073/pnas.95.17.9767

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

36 in total

1. The RNA polymerase II ternary complex cleaves the nascent transcript in a 3'----5' direction in the presence of elongation factor SII.

Authors: M G Izban; D S Luse
Journal: Genes Dev Date: 1992-07 Impact factor: 11.361

2. The RNA polymerase II elongation complex. Factor-dependent transcription elongation involves nascent RNA cleavage.

Authors: D Reines; P Ghanouni; Q Q Li; J Mote
Journal: J Biol Chem Date: 1992-08-05 Impact factor: 5.157

3. Transcription initiation by RNA polymerase II in vitro. At least two nucleotides must be added to form a stable ternary complex.

Authors: D S Luse; T Kochel; E D Kuempel; J A Coppola; H Cai
Journal: J Biol Chem Date: 1987-01-05 Impact factor: 5.157

4. Three transitions in the RNA polymerase II transcription complex during initiation.

Authors: F C Holstege; U Fiedler; H T Timmers
Journal: EMBO J Date: 1997-12-15 Impact factor: 11.598

5. Elongation by Drosophila RNA polymerase II. Transcription of 3'-extended DNA templates.

Authors: A E Sluder; D H Price; A L Greenleaf
Journal: J Biol Chem Date: 1988-07-15 Impact factor: 5.157

6. Factors involved in specific transcription by mammalian RNA polymerase II. Transcription factor IIS stimulates elongation of RNA chains.

Authors: D Reinberg; R G Roeder
Journal: J Biol Chem Date: 1987-03-05 Impact factor: 5.157

7. Abortive initiation by RNA polymerase II in vitro at the adenovirus 2 major late promoter.

Authors: D S Luse; G A Jacob
Journal: J Biol Chem Date: 1987-11-05 Impact factor: 5.157

Review 8. The selectivity of transcription.

Authors: M J Chamberlin
Journal: Annu Rev Biochem Date: 1974 Impact factor: 23.643

9. A block to elongation is largely responsible for decreased transcription of c-myc in differentiated HL60 cells.

Authors: D L Bentley; M Groudine
Journal: Nature Date: 1986 Jun 12-18 Impact factor: 49.962

10. Factors involved in specific transcription by mammalian RNA polymerase II. Factors IIE and IIF independently interact with RNA polymerase II.

Authors: O Flores; E Maldonado; D Reinberg
Journal: J Biol Chem Date: 1989-05-25 Impact factor: 5.157

26 in total

1. The general transcription factors IIA, IIB, IIF, and IIE are required for RNA polymerase II transcription from the human U1 small nuclear RNA promoter.

Authors: T C Kuhlman; H Cho; D Reinberg; N Hernandez
Journal: Mol Cell Biol Date: 1999-03 Impact factor: 4.272

2. Discrete promoter elements affect specific properties of RNA polymerase II transcription complexes.

Authors: J W Steinke; S J Kopytek; D O Peterson
Journal: Nucleic Acids Res Date: 2000-07-15 Impact factor: 16.971

3. Spt5 cooperates with human immunodeficiency virus type 1 Tat by preventing premature RNA release at terminator sequences.

Authors: Cyril F Bourgeois; Young Kyeung Kim; Mark J Churcher; Michelle J West; Jonathan Karn
Journal: Mol Cell Biol Date: 2002-02 Impact factor: 4.272

Promoter-proximal stalling results from the inability to recruit transcription factor IIH to the transcription complex and is a regulated event.

1. The RNA polymerase II ternary complex cleaves the nascent transcript in a 3'----5' direction in the presence of elongation factor SII.

2. The RNA polymerase II elongation complex. Factor-dependent transcription elongation involves nascent RNA cleavage.

3. Transcription initiation by RNA polymerase II in vitro. At least two nucleotides must be added to form a stable ternary complex.

4. Three transitions in the RNA polymerase II transcription complex during initiation.

5. Elongation by Drosophila RNA polymerase II. Transcription of 3'-extended DNA templates.

6. Factors involved in specific transcription by mammalian RNA polymerase II. Transcription factor IIS stimulates elongation of RNA chains.

7. Abortive initiation by RNA polymerase II in vitro at the adenovirus 2 major late promoter.

Review 8. The selectivity of transcription.

9. A block to elongation is largely responsible for decreased transcription of c-myc in differentiated HL60 cells.

10. Factors involved in specific transcription by mammalian RNA polymerase II. Factors IIE and IIF independently interact with RNA polymerase II.

1. The general transcription factors IIA, IIB, IIF, and IIE are required for RNA polymerase II transcription from the human U1 small nuclear RNA promoter.

2. Discrete promoter elements affect specific properties of RNA polymerase II transcription complexes.

3. Spt5 cooperates with human immunodeficiency virus type 1 Tat by preventing premature RNA release at terminator sequences.

4. Analysis of the open region of RNA polymerase II transcription complexes in the early phase of elongation.

5. Promoter clearance by RNA polymerase II is an extended, multistep process strongly affected by sequence.

6. Ubiquitination of RNA polymerase II large subunit signaled by phosphorylation of carboxyl-terminal domain.

7. An 8 nt RNA triggers a rate-limiting shift of RNA polymerase II complexes into elongation.

8. TFIIA plays a role in the response to oxidative stress.

9. A role for Chk1 in blocking transcriptional elongation of p21 RNA during the S-phase checkpoint.

10. The A- and B-type nuclear lamin networks: microdomains involved in chromatin organization and transcription.