Literature DB >> 9685509

A rapid and sensitive assay for histone acetyl-transferase activity.

S Ait-Si-Ali1, S Ramirez, P Robin, D Trouche, A Harel-Bellan.   

Abstract

Histone acetyl-transferases (HATs) seem to be key elements in the regulation of transcription. We have designed an enzymatic assay to quantify HAT enzymatic activity. In this assay, the substrate is a peptide corresponding to the 24 first amino acids of histone H4 which is coupled to biotin. After acetylation using [14C]acetyl-CoA, the peptide is purified on streptavidin beads and the associated radioactivity is measured. This assay is sensitive, rapid and convenient.

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Year:  1998        PMID： 9685509      PMCID： PMC147752          DOI： 10.1093/nar/26.16.3869

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  14 in total

1.  Use of phosph-cellulose paper disks for the assay of histone acetyltransferase.

Authors:  K Horiuchi; D Fujimoto
Journal:  Anal Biochem       Date:  1975-12       Impact factor: 3.365

2.  Identification of cellular proteins that can interact specifically with the T/E1A-binding region of the retinoblastoma gene product.

Authors:  W G Kaelin; D C Pallas; J A DeCaprio; F J Kaye; D M Livingston
Journal:  Cell       Date:  1991-02-08       Impact factor: 41.582

Review 3.  Histone acetylation and chromatin assembly: a single escort, multiple dances?

Authors:  S Y Roth; C D Allis
Journal:  Cell       Date:  1996-10-04       Impact factor: 41.582

4.  Rb interacts with histone deacetylase to repress transcription.

Authors:  R X Luo; A A Postigo; D C Dean
Journal:  Cell       Date:  1998-02-20       Impact factor: 41.582

5.  The transcriptional coactivators p300 and CBP are histone acetyltransferases.

Authors:  V V Ogryzko; R L Schiltz; V Russanova; B H Howard; Y Nakatani
Journal:  Cell       Date:  1996-11-29       Impact factor: 41.582

6.  The CBP co-activator is a histone acetyltransferase.

Authors:  A J Bannister; T Kouzarides
Journal:  Nature       Date:  1996 Dec 19-26       Impact factor: 49.962

Review 7.  Targeting chromatin disruption: Transcription regulators that acetylate histones.

Authors:  A P Wolffe; D Pruss
Journal:  Cell       Date:  1996-03-22       Impact factor: 41.582

8.  Tetrahymena histone acetyltransferase A: a homolog to yeast Gcn5p linking histone acetylation to gene activation.

Authors:  J E Brownell; J Zhou; T Ranalli; R Kobayashi; D G Edmondson; S Y Roth; C D Allis
Journal:  Cell       Date:  1996-03-22       Impact factor: 41.582

9.  An activity gel assay detects a single, catalytically active histone acetyltransferase subunit in Tetrahymena macronuclei.

Authors:  J E Brownell; C D Allis
Journal:  Proc Natl Acad Sci U S A       Date:  1995-07-03       Impact factor: 11.205

Review 10.  Histone acetylation and control of gene expression.

Authors:  B M Turner
Journal:  J Cell Sci       Date:  1991-05       Impact factor: 5.285
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  18 in total

1.  Interaction between acetylated MyoD and the bromodomain of CBP and/or p300.

Authors:  A Polesskaya; I Naguibneva; A Duquet; E Bengal; P Robin; A Harel-Bellan
Journal:  Mol Cell Biol       Date:  2001-08       Impact factor: 4.272

2.  Functional analysis of the p300 acetyltransferase domain: the PHD finger of p300 but not of CBP is dispensable for enzymatic activity.

Authors:  L Bordoli; S Hüsser; U Lüthi; M Netsch; H Osmani; R Eckner
Journal:  Nucleic Acids Res       Date:  2001-11-01       Impact factor: 16.971

3.  A non-isotopic in vitro assay for histone acetylation.

Authors:  David Kuninger; James Lundblad; Anthony Semirale; Peter Rotwein
Journal:  J Biotechnol       Date:  2007-07-12       Impact factor: 3.307

4.  Effective Quenchers Are Required to Eliminate the Interference of Substrate: Cofactor Binding in the HAT Scintillation Proximity Assay.

Authors:  Liza Ngo; Jiang Wu; Chao Yang; Yujun George Zheng
Journal:  Assay Drug Dev Technol       Date:  2015-05-28       Impact factor: 1.738

5.  CBP/p300 and muscle differentiation: no HAT, no muscle.

Authors:  A Polesskaya; I Naguibneva; L Fritsch; A Duquet; S Ait-Si-Ali; P Robin; A Vervisch; L L Pritchard; P Cole; A Harel-Bellan
Journal:  EMBO J       Date:  2001-12-03       Impact factor: 11.598

6.  Ultrasensitive electrochemiluminescence immunosensor for the transcriptional co-activator p300 by using a graphene oxide monolayer and tetrahedral DNA-mediated signal amplification.

Authors:  Yufang Hu; Qingqing Zhang; Dandan Hu; Jiao Wang; Jiajia Rao; Lihua Xu; Zhiyong Guo; Sui Wang; Xin Liu; Shiyun Tang; Qinpeng Shen
Journal:  Mikrochim Acta       Date:  2019-05-02       Impact factor: 5.833

7.  Physical association between the histone acetyl transferase CBP and a histone methyl transferase.

Authors:  L Vandel; D Trouche
Journal:  EMBO Rep       Date:  2001-01       Impact factor: 8.807

8.  Mitogen-regulated RSK2-CBP interaction controls their kinase and acetylase activities.

Authors:  K Merienne; S Pannetier; A Harel-Bellan; P Sassone-Corsi
Journal:  Mol Cell Biol       Date:  2001-10       Impact factor: 4.272

9.  Msx3 protein recruits histone deacetylase to down-regulate the Msx1 promoter.

Authors:  R Mehra-Chaudhary; H Matsui; R Raghow
Journal:  Biochem J       Date:  2001-01-01       Impact factor: 3.857

10.  Transcriptional deregulation of the keratin 18 gene in human colon carcinoma cells results from an altered acetylation mechanism.

Authors:  Philippe Prochasson; Cécile Delouis; Olivier Brison
Journal:  Nucleic Acids Res       Date:  2002-08-01       Impact factor: 16.971
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