| Literature DB >> 9685506 |
M F Wolschek1, F Narendja, J Karlseder, C P Kubicek, C Scazzocchio, J Strauss.
Abstract
The method described here allows the detection of protein-DNA interactions in vivo in filamentous fungi. We outline culture conditions and conditions of in vivo methylation that permit uniform modification of all cells in an apically growing, non-uniform organism, and subsequent visualization of protected areas by ligation-mediated PCR.Mesh:
Substances:
Year: 1998 PMID: 9685506 PMCID: PMC147760 DOI: 10.1093/nar/26.16.3862
Source DB: PubMed Journal: Nucleic Acids Res ISSN: 0305-1048 Impact factor: 16.971