Literature DB >> 9679295

Solvent exchange rates of side-chain amide protons in proteins.

P Rajagopal1, B E Jones, R E Klevit.   

Abstract

Solvent exchange rates and temperature coefficients for Asn/Gln side-chain amide protons have been measured in Escherichia coli HPr. The protons of the eight side-chain amide groups (two Asn and six Gln) exhibit varying exchange rates which are slower than some of the fast exchanging backbone amide protons. Differences in exchange rates of the E and Z protons of the same side-chain amide group are obtained by measuring exchange rates at pH values > 8. An NOE between a side-chain amide proton and a bound water molecule was also observed.

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Year:  1998        PMID： 9679295     DOI： 10.1023/a:1008296932751

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  14 in total

1.  Temperature dependence of 1H chemical shifts in proteins.

Authors:  N J Baxter; M P Williamson
Journal:  J Biomol NMR       Date:  1997-06       Impact factor: 2.835

2.  H2NCO-E.COSY, a simple method for the sterospecific assignment of side-chain amide protons in proteins.

Authors:  F Löhr; H Rüterjans
Journal:  J Magn Reson       Date:  1997-01       Impact factor: 2.229

3.  Measurement of the exchange rates of rapidly exchanging amide protons: application to the study of calmodulin and its complex with a myosin light chain kinase fragment.

Authors:  S Spera; M Ikura; A Bax
Journal:  J Biomol NMR       Date:  1991-07       Impact factor: 2.835

4.  NMRPipe: a multidimensional spectral processing system based on UNIX pipes.

Authors:  F Delaglio; S Grzesiek; G W Vuister; G Zhu; J Pfeifer; A Bax
Journal:  J Biomol NMR       Date:  1995-11       Impact factor: 2.835

5.  Primary structure effects on peptide group hydrogen exchange.

Authors:  Y Bai; J S Milne; L Mayne; S W Englander
Journal:  Proteins       Date:  1993-09

6.  Reexamination of the secondary and tertiary structure of histidine-containing protein from Escherichia coli by homonuclear and heteronuclear NMR spectroscopy.

Authors:  P K Hammen; E B Waygood; R E Klevit
Journal:  Biochemistry       Date:  1991-12-24       Impact factor: 3.162

7.  The 1.6 A structure of histidine-containing phosphotransfer protein HPr from Streptococcus faecalis.

Authors:  Z Jia; M Vandonselaar; W Hengstenberg; J W Quail; L T Delbaere
Journal:  J Mol Biol       Date:  1994-03-11       Impact factor: 5.469

8.  Backbone dynamics of a free and phosphopeptide-complexed Src homology 2 domain studied by 15N NMR relaxation.

Authors:  N A Farrow; R Muhandiram; A U Singer; S M Pascal; C M Kay; G Gish; S E Shoelson; T Pawson; J D Forman-Kay; L E Kay
Journal:  Biochemistry       Date:  1994-05-17       Impact factor: 3.162

9.  Measurement of amide proton exchange rates and NOEs with water in 13C/15N-enriched calcineurin B.

Authors:  S Grzesiek; A Bax
Journal:  J Biomol NMR       Date:  1993-11       Impact factor: 2.835

10.  Side-chain dynamics of a detergent-solubilized membrane protein: measurement of tryptophan and glutamine hydrogen-exchange rates in M13 coat protein by 1H NMR spectroscopy.

Authors:  J D O'Neil; B D Sykes
Journal:  Biochemistry       Date:  1989-08-08       Impact factor: 3.162
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  1 in total

1.  A Second Backbone: The Contribution of a Buried Asparagine Ladder to the Global and Local Stability of a Leucine-Rich Repeat Protein.

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Journal:  Biochemistry       Date:  2019-08-06       Impact factor: 3.162
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