| Literature DB >> 9673287 |
Abstract
The green fluorescent protein (GFP) gene was expressed on a plasmid in B. henselae, and GFP-expressing bacteria were visualized by fluorescence microscopy. HEp-2 cells infected with GFP-expressing bacteria were separated from uninfected cells with a fluorescence activated cell sorter. Promoter fusions of B. henselae chromosomal DNA to gfp were examined by flow cytometry, and a B. henselae groEL promoter fusion which induced expression at 37 degreesC was isolated.Entities:
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Year: 1998 PMID: 9673287 PMCID: PMC108464
Source DB: PubMed Journal: Infect Immun ISSN: 0019-9567 Impact factor: 3.441