Literature DB >> 9671124

Rapid fluorescence-based measurement of neutrophil migration in vitro.

C W Frevert1, V A Wong, R B Goodman, R Goodwin, T R Martin.   

Abstract

We have standardized a new chemotaxis chamber that uses fluorescence as the cellular marker for the measurement of leukocyte migration in vitro in disposable 96-well microplates. This new fluorescence-based assay is a robust assay because filter pore size, cell density, filter composition, and filter thickness do not affect PMN migration towards interleukin-8 or the complement fragment, C5a. When compared to two separate chemotaxis assays in which the migrated cells are counted visually, the fluorescence-based assay was more rapid, less labor intensive, and more sensitive. This new assay is a significant advance in the measurement of leukocyte migration in vitro.

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Year:  1998        PMID： 9671124     DOI： 10.1016/s0022-1759(98)00016-7

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  42 in total

1.  Pre-B-cell colony-enhancing factor is a secreted cytokine-like protein from the human amniotic epithelium.

Authors:  Simona Ognjanovic; Tercia L Ku; Gillian D Bryant-Greenwood
Journal:  Am J Obstet Gynecol       Date:  2005-07       Impact factor: 8.661

2.  Virus-infected alveolar epithelial cells direct neutrophil chemotaxis and inhibit their apoptosis.

Authors:  Joanna P Rzepka; Anoria K Haick; Tanya A Miura
Journal:  Am J Respir Cell Mol Biol       Date:  2012-02-03       Impact factor: 6.914

3.  Microglial cell migration stimulated by ATP and C5a involve distinct molecular mechanisms: quantification of migration by a novel near-infrared method.

Authors:  Aaron M Miller; Nephi Stella
Journal:  Glia       Date:  2009-06       Impact factor: 7.452

4.  Myristoylated Alanine Rich C Kinase Substrate (MARCKS) is essential to β2-integrin dependent responses of equine neutrophils.

Authors:  Mary K Sheats; Kimberly C Pescosolido; Ethan M Hefner; Eui Jae Sung; Kenneth B Adler; Samuel L Jones
Journal:  Vet Immunol Immunopathol       Date:  2014-05-02       Impact factor: 2.046

5.  Extracellular ATP mediates the late phase of neutrophil recruitment to the lung in murine models of acute lung injury.

Authors:  Dilip Shah; Freddy Romero; William Stafstrom; Michelle Duong; Ross Summer
Journal:  Am J Physiol Lung Cell Mol Physiol       Date:  2013-11-27       Impact factor: 5.464

6.  Developmental changes in circulating IL-8/CXCL8 isoforms in neonates.

Authors:  Akhil Maheshwari; Nikolai N Voitenok; Svetlana Akalovich; Sadiq S Shaik; David A Randolph; Brian Sims; Rakesh P Patel; Cheryl R Killingsworth; Michael B Fallon; Robin K Ohls
Journal:  Cytokine       Date:  2009-02-15       Impact factor: 3.861

7.  Prasugrel metabolites inhibit neutrophil functions.

Authors:  Elisabetta Liverani; Mario C Rico; Analia E Garcia; Laurie E Kilpatrick; Satya P Kunapuli
Journal:  J Pharmacol Exp Ther       Date:  2012-10-24       Impact factor: 4.030

8.  Tissue inhibitor of metalloproteinases 3 regulates resolution of inflammation following acute lung injury.

Authors:  Sean E Gill; Isham Huizar; Eli M Bench; Samuel W Sussman; Ying Wang; Rama Khokha; William C Parks
Journal:  Am J Pathol       Date:  2009-12-11       Impact factor: 4.307

9.  Duffy antigen facilitates movement of chemokine across the endothelium in vitro and promotes neutrophil transmigration in vitro and in vivo.

Authors:  Janet S Lee; Charles W Frevert; Mark M Wurfel; Stephen C Peiper; Venus A Wong; Kimberley K Ballman; John T Ruzinski; Johng S Rhim; Thomas R Martin; Richard B Goodman
Journal:  J Immunol       Date:  2003-05-15       Impact factor: 5.422

10.  Differential effect of saturated and unsaturated free fatty acids on the generation of monocyte adhesion and chemotactic factors by adipocytes: dissociation of adipocyte hypertrophy from inflammation.

Authors:  Chang Yeop Han; Atil Y Kargi; Mohamed Omer; Christina K Chan; Martin Wabitsch; Kevin D O'Brien; Thomas N Wight; Alan Chait
Journal:  Diabetes       Date:  2009-11-23       Impact factor: 9.461
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