Literature DB >> 9668349

Use of BODIPY-labeled sphingolipids to study membrane traffic along the endocytic pathway.

R E Pagano1, C S Chen.   

Abstract

In this chapter we discuss the use of BODIPY-labeled sphingolipids to study lipid transport along the endocytic pathway of cultured mammalian cells. The unique spectral properties of the BODIPY fluorophore allow the investigator to distinguish various populations of labeled endosome and lysosomes within the living cell by fluorescence microscopy, and in conjunction with quantitative fluorescence microscopy, to estimate the concentration of these lipids in different intracellular compartments. This methodology is particularly useful for visualizing the accumulation of lipids in the lysosomes of storage disease fibroblasts and may provide a useful method for screening various agents that abrogate this accumulation.

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Year:  1998        PMID： 9668349     DOI： 10.1111/j.1749-6632.1998.tb09668.x

Source DB:  PubMed          Journal:  Ann N Y Acad Sci        ISSN： 0077-8923            Impact factor:   5.691


  22 in total

1.  A pitfall in using BODIPY dyes to label lipid droplets for fluorescence microscopy.

Authors:  Yuki Ohsaki; Yuki Shinohara; Michitaka Suzuki; Toyoshi Fujimoto
Journal:  Histochem Cell Biol       Date:  2010-02-27       Impact factor: 4.304

2.  Abnormal transport along the lysosomal pathway in mucolipidosis, type IV disease.

Authors:  C S Chen; G Bach; R E Pagano
Journal:  Proc Natl Acad Sci U S A       Date:  1998-05-26       Impact factor: 11.205

3.  Synthesis of reference standards to enable single cell metabolomic studies of tetramethylrhodamine-labeled ganglioside GM1.

Authors:  E Andreas Larsson; Ulf Olsson; Colin D Whitmore; Rita Martins; Guido Tettamanti; Ronald L Schnaar; Norman J Dovichi; Monica M Palcic; Ole Hindsgaul
Journal:  Carbohydr Res       Date:  2006-10-07       Impact factor: 2.104

4.  Probing endocytosis from the enterocyte brush border using fluorescent lipophilic dyes: lipid sorting at the apical cell surface.

Authors:  E Michael Danielsen
Journal:  Histochem Cell Biol       Date:  2014-12-20       Impact factor: 4.304

5.  Screening toxicity study in young carp (Cyprinus carpio L.) on feed amended with fumonisin B1.

Authors:  Stjepan Pepeljnjak; Zdravko Petrinec; Sanja Kovacic; Maja Segvic
Journal:  Mycopathologia       Date:  2003       Impact factor: 2.574

6.  Real-time analysis of endosomal lipid transport by live cell scintillation proximity assay.

Authors:  Walter Stockinger; Adam B Castoreno; Yan Wang; Joanne C Pagnon; Axel Nohturfft
Journal:  J Lipid Res       Date:  2004-08-16       Impact factor: 5.922

7.  Ceramidase regulates synaptic vesicle exocytosis and trafficking.

Authors:  Jeffrey Rohrbough; Emma Rushton; Laura Palanker; Elvin Woodruff; Heinrich J G Matthies; Usha Acharya; Jairaj K Acharya; Kendal Broadie
Journal:  J Neurosci       Date:  2004-09-08       Impact factor: 6.167

8.  Role of endocytosis in the internalization of spermidine-C(2)-BODIPY, a highly fluorescent probe of polyamine transport.

Authors:  Denis Soulet; Laurence Covassin; Mohammadi Kaouass; René Charest-Gaudreault; Marie Audette; Richard Poulin
Journal:  Biochem J       Date:  2002-10-15       Impact factor: 3.857

Review 9.  Cholesterol regulation of rab-mediated sphingolipid endocytosis.

Authors:  Miwa Takahashi; Toshihide Kobayashi
Journal:  Glycoconj J       Date:  2008-10-08       Impact factor: 2.916

10.  DNA modification of live cell surface.

Authors:  Grigory G Borisenko; Marina A Zaitseva; Andrey N Chuvilin; Galina E Pozmogova
Journal:  Nucleic Acids Res       Date:  2009-01-21       Impact factor: 16.971
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