Literature DB >> 20191286

A pitfall in using BODIPY dyes to label lipid droplets for fluorescence microscopy.

Yuki Ohsaki1, Yuki Shinohara, Michitaka Suzuki, Toyoshi Fujimoto.   

Abstract

The lipid droplet (LD) has become a focus of intense research. Fluorescence labeling is indispensable for the cell biological analysis of the LD, and a lipophilic fluorescence dye, BODIPY 493/503, which emits bright green fluorescence has been used extensively for LD labeling. The dye is convenient for double fluorescence labeling, but we noticed that it emits red fluorescence under certain conditions, which could lead to erroneous interpretations. We propose a protocol to preclude such a possibility.

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Year:  2010        PMID： 20191286     DOI： 10.1007/s00418-010-0678-x

Source DB:  PubMed          Journal:  Histochem Cell Biol        ISSN： 0948-6143            Impact factor:   4.304


  15 in total

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Review 10.  Lipid droplets: a classic organelle with new outfits.

Authors:  Toyoshi Fujimoto; Yuki Ohsaki; Jinglei Cheng; Michitaka Suzuki; Yuki Shinohara
Journal:  Histochem Cell Biol       Date:  2008-06-11       Impact factor: 4.304
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  19 in total

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Review 5.  Synthetic fluorescent probes for monovalent copper.

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