Literature DB >> 9666736

Complete and rapid peptide and glycopeptide mapping of mouse monoclonal antibody by LC/MS/MS using ion trap mass spectrometry.

K Hirayama1, R Yuji, N Yamada, K Kato, Y Arata, I Shimada.   

Abstract

Complete and rapid peptide and glycopeptide mapping of a mouse monoclonal immunoglobulin (IgG2b) were carried out by liquid chromatography/electrospray ionization ion trap-mass spectrometry/mass spectrometry (LC/ ESI IT-MS/MS). It was possible to obtain spectra of a minor glycopeptide at a quantity as low as 1.8 pmol. Reduced and carboxymethylated mouse antidansyl monoclonal IgG2b (RCM-IgG2b) was digested with lysyl-endopeptidase. Proteolytic peptides were subjected to capillary HPLC separation followed by analysis with an ion trap mass spectrometer. The complete amino acid sequence of the IgG2b was characterized by using LC/ ESI IT-MS/MS. The structures of 12 different types of O-linked oligosaccharides attached to Thr-221AH in the hinge region and those of three major types of N-linked oligosaccharides attached to Asn-297H have been characterized.

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Year:  1998        PMID： 9666736     DOI： 10.1021/ac9712153

Source DB:  PubMed          Journal:  Anal Chem        ISSN： 0003-2700            Impact factor:   6.986


  8 in total

1.  Determination of primary structure and microheterogeneity of a beta-amyloid plaque-specific antibody using high-performance LC-tandem mass spectrometry.

Authors:  Irina Perdivara; Leesa Deterding; Adrian Moise; Kenneth B Tomer; Michael Przybylski
Journal:  Anal Bioanal Chem       Date:  2008-03-28       Impact factor: 4.142

2.  Mass Spectrometry-Based Methods for Immunoglobulin G N-Glycosylation Analysis.

Authors:  Siniša Habazin; Jerko Štambuk; Jelena Šimunović; Toma Keser; Genadij Razdorov; Mislav Novokmet
Journal:  Exp Suppl       Date:  2021

3.  Lectin and Liquid Chromatography-Based Methods for Immunoglobulin (G) Glycosylation Analysis.

Authors:  Tea Petrović; Irena Trbojević-Akmačić
Journal:  Exp Suppl       Date:  2021

4.  Rapid Highly-Efficient Digestion and Peptide Mapping of Adeno-Associated Viruses.

Authors:  Estee Naggar Toole; Craig Dufresne; Somak Ray; Alexander Schwann; Ken Cook; Alexander R Ivanov
Journal:  Anal Chem       Date:  2021-07-22       Impact factor: 6.986

5.  On-line capillary liquid chromatography tandem mass spectrometry on an ion trap/reflectron time-of-flight mass spectrometer using the sequence tag database search approach for peptide sequencing and protein identification.

Authors:  P Huang; D B Wall; S Parus; D M Lubman
Journal:  J Am Soc Mass Spectrom       Date:  2000-02       Impact factor: 3.262

6.  Developing a medium combination to attain similar glycosylation profile to originator by DoE and cluster analysis method.

Authors:  Jian Xu; Zhihui Shao; Zhanqing Wang; Yingfeng Huang; Xun Zou; Yaling Shen
Journal:  Sci Rep       Date:  2021-03-29       Impact factor: 4.379

7.  Comparative Glycopeptide Analysis for Protein Glycosylation by Liquid Chromatography and Tandem Mass Spectrometry: Variation in Glycosylation Patterns of Site-Directed Mutagenized Glycoprotein.

Authors:  Young Hye Hahm; Sung Ho Hahm; Hyoun Young Jo; Yeong Hee Ahn
Journal:  Int J Anal Chem       Date:  2018-09-02       Impact factor: 1.885

8.  Site-specific N-glycosylation analysis: matrix-assisted laser desorption/ionization quadrupole-quadrupole time-of-flight tandem mass spectral signatures for recognition and identification of glycopeptides.

Authors:  Oleg Krokhin; Werner Ens; Kenneth G Standing; John Wilkins; Hélène Perreault
Journal:  Rapid Commun Mass Spectrom       Date:  2004       Impact factor: 2.419
  8 in total

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