Literature DB >> 9666016

Screening of stool samples for identification of vancomycin-resistant Enterococcus isolates should include the methyl-alpha-D-glucopyranoside test to differentiate nonmotile Enterococcus gallinarum from E. faecium.

C Y Turenne1, D J Hoban, J A Karlowsky, G G Zhanel, A M Kabani.   

Abstract

The methyl-alpha-D-glucopyranoside (MDG) test has been shown to be superior to motility testing in differentiating Enterococcus faecium from E. gallinarum. In the present study, 33 vancomycin-resistant enterococcus (VRE) isolates collected as part of a stool surveillance study were compared by using motility and MDG. Motility testing identified all 33 isolates as E. faecium, whereas MDG identified 11 of the 33 isolates as nonmotile E. gallinarum. The MDG results were confirmed by sequencing the 16S rDNA V6-to-V8 region. We conclude that the MDG test is a necessary component of routine VRE screening.

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Year:  1998        PMID： 9666016      PMCID： PMC105042     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  14 in total

1.  Vancomycin-resistant enterococci (VRE) in Canada - Results of the Canadian Nosocomial Infection Surveillance Program 1996 VRE point prevalence surveillance project.

Authors:  M E Ofner-Agostini; J Conly; S Paton; A Kureishi; L Nicolle; M Mulvey; W Johnson; L Johnston
Journal:  Can J Infect Dis       Date:  1997-03

2.  Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR.

Authors:  S Dutka-Malen; S Evers; P Courvalin
Journal:  J Clin Microbiol       Date:  1995-01       Impact factor: 5.948

3.  Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli.

Authors:  J Brosius; M L Palmer; P J Kennedy; H F Noller
Journal:  Proc Natl Acad Sci U S A       Date:  1978-10       Impact factor: 11.205

4.  Acidification of methyl-alpha-D-glucopyranoside: a useful test to differentiate Enterococcus casseliflavus and Enterococcus gallinarum from Enterococcus faecium species group and from Enterococcus faecalis.

Authors:  L A Devriese; B Pot; K Kersters; S Lauwers; F Haesebrouck
Journal:  J Clin Microbiol       Date:  1996-10       Impact factor: 5.948

Review 5.  Genetics and mechanisms of glycopeptide resistance in enterococci.

Authors:  M Arthur; P Courvalin
Journal:  Antimicrob Agents Chemother       Date:  1993-08       Impact factor: 5.191

6.  Vancomycin-resistant enterococcal bacteremia: natural history and attributable mortality.

Authors:  M B Edmond; J F Ober; J D Dawson; D L Weinbaum; R P Wenzel
Journal:  Clin Infect Dis       Date:  1996-12       Impact factor: 9.079

7.  Major trends in the microbial etiology of nosocomial infection.

Authors:  D R Schaberg; D H Culver; R P Gaynes
Journal:  Am J Med       Date:  1991-09-16       Impact factor: 4.965

8.  DNA hybridization and contour-clamped homogeneous electric field electrophoresis for identification of enterococci to the species level.

Authors:  S Donabedian; J W Chow; D M Shlaes; M Green; M J Zervos
Journal:  J Clin Microbiol       Date:  1995-01       Impact factor: 5.948

9.  Comparison of pigment production and motility tests with PCR for reliable identification of intrinsically vancomycin-resistant enterococci.

Authors:  C P Cartwright; F Stock; G A Fahle; V J Gill
Journal:  J Clin Microbiol       Date:  1995-07       Impact factor: 5.948

Review 10.  Vancomycin-resistant enterococci.

Authors:  A S Gin; G G Zhanel
Journal:  Ann Pharmacother       Date:  1996-06       Impact factor: 3.154
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  8 in total

1.  Evaluation of 15 motility media and a direct microscopic method for detection of motility in enterococci.

Authors:  Kenneth Van Horn; Clara Tóth; Reiko Kariyama; Ritsuko Mitsuhata; Hiromi Kumon
Journal:  J Clin Microbiol       Date:  2002-07       Impact factor: 5.948

2.  Evaluation of the revised MicroScan dried overnight gram-positive identification panel to identify Enterococcus species.

Authors:  P C Iwen; M E Rupp; P C Schreckenberger; S H Hinrichs
Journal:  J Clin Microbiol       Date:  1999-11       Impact factor: 5.948

3.  Evaluation of D-xylose and 1% methyl-alpha-D-glucopyranoside fermentation tests for distinguishing Enterococcus gallinarum from Enterococcus faecium.

Authors:  D K Chen; L Pearce; A McGeer; D E Low; B M Willey
Journal:  J Clin Microbiol       Date:  2000-10       Impact factor: 5.948

4.  Nitrofurantoin is active against vancomycin-resistant enterococci.

Authors:  G G Zhanel; D J Hoban; J A Karlowsky
Journal:  Antimicrob Agents Chemother       Date:  2001-01       Impact factor: 5.191

5.  Low prevalence of VRE gastrointestinal colonization of hospitalized patients in Manitoba tertiary care and community hospitals.

Authors:  G G Zhanel; G K Harding; S Rosser; D J Hoban; J A Karlowsky; M Alfa; A Kabani; J Embil; A Gin; T Williams; L E Nicolle
Journal:  Can J Infect Dis       Date:  2000-01

6.  Proficiency of clinical laboratories in and near Monterrey, Mexico, to detect vancomycin-resistant enterococci.

Authors:  L C McDonald; L R Garza; W R Jarvis
Journal:  Emerg Infect Dis       Date:  1999 Jan-Feb       Impact factor: 6.883

7.  Vancomycin-resistant enterococci in a Chinese hospital.

Authors:  S Li; Z Zhang; Z H Mi
Journal:  Curr Microbiol       Date:  2007-07-04       Impact factor: 2.343

8.  Is the mazEF toxin-antitoxin system responsible for vancomycin resistance in clinical isolates of Enterococcus faecalis?

Authors:  Nourkhoda Sadeghifard; Sara Soheili; Zamberi Sekawi; Sobhan Ghafourian
Journal:  GMS Hyg Infect Control       Date:  2014-03-07
  8 in total

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