High-performance liquid chromatography analysis of Bobel-24 in biological samples for pharmacokinetic, metabolic and tissue distribution studies.

A rapid and simple HPLC method is described for the determination of Bobel-24 (2,4,6-triiodophenol) and other iodinated derivatives in biological samples. The sample preparation was liquid-liquid extraction before injection onto the HPLC system. 2,6-Diiodo-4-methylphenol was used as internal standard. Separation was obtained using a reversed-phase column under isocratic conditions. The mobile phase consisted of water-acetonitrile (62:38). 2,4,6-Triiodophenol was detected at 277 nm. This method was used for Bobel-24 determination in plasma, urine, synovial liquid and different tissues. The assay was applied to pharmacokinetic studies in dog and horse plasma and different dog tissues for tissue distribution profiles toxicological and metabolic studies. In addition, this method for biological samples can be applied to non-biological samples such as pharmaceutical formulations in stability studies and quality control assays.