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Literature DB >> 9649430

Aut2p and Aut7p, two novel microtubule-associated proteins are essential for delivery of autophagic vesicles to the vacuole.

T Lang¹, E Schaeffeler, D Bernreuther, M Bredschneider, D H Wolf, M Thumm.

Abstract

AUT2 and AUT7, two novel genes essential for autophagocytosis in the yeast Saccharomyces cerevisiae were isolated. AUT7 was identified as a low copy suppressor of autophagic defects in aut2-1 cells. Aut7p is a homologue of the rat microtubule-associated protein (MAP) light chain 3 (LC3). Aut2p and Aut7p interact physically. Aut7p is attached to microtubules via Aut2p, which interacts with tubulins Tub1p and Tub2p. aut2- and aut7-deleted cells are unable to deliver autophagic vesicles and the precursor of aminopeptidase I to the vacuole. Double membrane-layered autophagosome-like vesicles accumulate in the cytoplasm of these cells. Our findings suggest that microtubules and an attached protein complex of Aut2p and Aut7p are involved in the delivery of autophagic vesicles to the vacuole.

Entities: Gene Species

Mesh：

Substances：

Year: 1998 PMID： 9649430 PMCID： PMC1170696 DOI： 10.1093/emboj/17.13.3597

Source DB: PubMed Journal: EMBO J ISSN： 0261-4189 Impact factor: 11.598

37 in total

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Authors: P Codogno; E Ogier-Denis; J J Houri
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2. Isolation and characterization of conditional-lethal mutations in the TUB1 alpha-tubulin gene of the yeast Saccharomyces cerevisiae.

Authors: P J Schatz; F Solomon; D Botstein
Journal: Genetics Date: 1988-11 Impact factor: 4.562

3. A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector.

Authors: M D Rose; P Novick; J H Thomas; D Botstein; G R Fink
Journal: Gene Date: 1987 Impact factor: 3.688

Review 4. Structural aspects of autophagy.

Authors: P O Seglen; T O Berg; H Blankson; M Fengsrud; I Holen; P E Strømhaug
Journal: Adv Exp Med Biol Date: 1996 Impact factor: 2.622

5. Genetic and phenotypic overlap between autophagy and the cytoplasm to vacuole protein targeting pathway.

Authors: T M Harding; A Hefner-Gravink; M Thumm; D J Klionsky
Journal: J Biol Chem Date: 1996-07-26 Impact factor: 5.157

6. A novel genetic system to detect protein-protein interactions.

Authors: S Fields; O Song
Journal: Nature Date: 1989-07-20 Impact factor: 49.962

7. The molecular structure of microtubule-associated protein 1A (MAP1A) in vivo and in vitro. An immunoelectron microscopy and quick-freeze, deep-etch study.

Authors: Y Shiomura; N Hirokawa
Journal: J Neurosci Date: 1987-05 Impact factor: 6.167

8. Isolation of autophagocytosis mutants of Saccharomyces cerevisiae.

Authors: M Thumm; R Egner; B Koch; M Schlumpberger; M Straub; M Veenhuis; D H Wolf
Journal: FEBS Lett Date: 1994-08-01 Impact factor: 4.124

9. Tracing intracellular proteolytic pathways. Proteolysis of fatty acid synthase and other cytoplasmic proteins in the yeast Saccharomyces cerevisiae.

Authors: R Egner; M Thumm; M Straub; A Simeon; H J Schüller; D H Wolf
Journal: J Biol Chem Date: 1993-12-25 Impact factor: 5.157

10. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae.

Authors: A Wach; A Brachat; R Pöhlmann; P Philippsen
Journal: Yeast Date: 1994-12 Impact factor: 3.239

92 in total

1. LB-AUT7, a novel symbiosis-regulated gene from an ectomycorrhizal fungus, Laccaria bicolor, is functionally related to vesicular transport and autophagocytosis.

Authors: S J Kim; D Bernreuther; M Thumm; G K Podila
Journal: J Bacteriol Date: 1999-03 Impact factor: 3.490

Aut2p and Aut7p, two novel microtubule-associated proteins are essential for delivery of autophagic vesicles to the vacuole.

Review 1. Signal transduction pathways in macroautophagy.

2. Isolation and characterization of conditional-lethal mutations in the TUB1 alpha-tubulin gene of the yeast Saccharomyces cerevisiae.

3. A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector.

Review 4. Structural aspects of autophagy.

5. Genetic and phenotypic overlap between autophagy and the cytoplasm to vacuole protein targeting pathway.

6. A novel genetic system to detect protein-protein interactions.

7. The molecular structure of microtubule-associated protein 1A (MAP1A) in vivo and in vitro. An immunoelectron microscopy and quick-freeze, deep-etch study.

8. Isolation of autophagocytosis mutants of Saccharomyces cerevisiae.

9. Tracing intracellular proteolytic pathways. Proteolysis of fatty acid synthase and other cytoplasmic proteins in the yeast Saccharomyces cerevisiae.

10. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae.

1. LB-AUT7, a novel symbiosis-regulated gene from an ectomycorrhizal fungus, Laccaria bicolor, is functionally related to vesicular transport and autophagocytosis.

2. Apg7p/Cvt2p is required for the cytoplasm-to-vacuole targeting, macroautophagy, and peroxisome degradation pathways.

Review 3. Autophagy as a regulated pathway of cellular degradation.

4. LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing.

Review 5. The molecular mechanism of autophagy.

6. New thoughts regarding Atg8 and ubiquitination.

7. KIF1A/UNC-104 Transports ATG-9 to Regulate Neurodevelopment and Autophagy at Synapses.

Review 8. Autophagy: molecular machinery for self-eating.

9. Two newly identified sites in the ubiquitin-like protein Atg8 are essential for autophagy.

10. Visualizing the autophagy pathway in avian cells and its application to studying infectious bronchitis virus.