Literature DB >> 963219

Numerical study of the Johnston-Ogston effect in two-component systems.

J J Correia, M L Johnson, G H Weiss, D A Yphantis.   

Abstract

Numerical solutions of the Lamm equation are presented for systems exhibiting the Johnston--Ogston effect. From these solutions it is apparent that the movement of the maxima of the concentration gradient curves reflects the sedimentation velocity of the slow or fast components in their appropriate plateaus. A simple generalization of the Johnston--Ogston analysis is presented, valid for all centrifugation times in a radial field and sector shaped cell provided only that there exist both a plateau of the slow component by itself and the mixed plateau with both slow and fast components present.

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Year:  1976        PMID: 963219     DOI: 10.1016/0301-4622(76)80038-5

Source DB:  PubMed          Journal:  Biophys Chem        ISSN: 0301-4622            Impact factor:   2.352


  7 in total

1.  Identification and interpretation of complexity in sedimentation velocity boundaries.

Authors:  B Demeler; H Saber; J C Hansen
Journal:  Biophys J       Date:  1997-01       Impact factor: 4.033

2.  Sedimentation Velocity Analysis with Fluorescence Detection of Mutant Huntingtin Exon 1 Aggregation in Drosophila melanogaster and Caenorhabditis elegans.

Authors:  Surin A Kim; Victoria F D'Acunto; Bashkim Kokona; Jennifer Hofmann; Nicole R Cunningham; Emily M Bistline; F Jay Garcia; Nabeel M Akhtar; Susanna H Hoffman; Seema H Doshi; Kathleen M Ulrich; Nicholas M Jones; Nancy M Bonini; Christine M Roberts; Christopher D Link; Thomas M Laue; Robert Fairman
Journal:  Biochemistry       Date:  2017-08-24       Impact factor: 3.162

3.  NUTS and BOLTS: applications of fluorescence-detected sedimentation.

Authors:  Rachel R Kroe; Thomas M Laue
Journal:  Anal Biochem       Date:  2008-12-06       Impact factor: 3.365

4.  Structural and hydrodynamic analysis of a novel drug delivery vector: ELP[V5G3A2-150].

Authors:  Daniel F Lyons; Vu Le; Gene L Bidwell; Wolfgang H Kramer; Edwin A Lewis; Drazen Raucher; John J Correia
Journal:  Biophys J       Date:  2013-05-07       Impact factor: 4.033

5.  Analysis of antibody aggregate content at extremely high concentrations using sedimentation velocity with a novel interference optics.

Authors:  Kristian Schilling; Frank Krause
Journal:  PLoS One       Date:  2015-03-24       Impact factor: 3.240

6.  Clinically Relevant Insulin Degludec and its Interaction with Polysaccharides: A Biophysical Examination.

Authors:  Shahwar Imran Jiwani; Sha Huang; Oritsegidenene Beji; Philemon Gyasi-Antwi; Richard B Gillis; Gary G Adams
Journal:  Polymers (Basel)       Date:  2020-02-09       Impact factor: 4.329

7.  Analysis of nonideality: insights from high concentration simulations of sedimentation velocity data.

Authors:  J J Correia; R T Wright; P J Sherwood; W F Stafford
Journal:  Eur Biophys J       Date:  2020-11-06       Impact factor: 1.733

  7 in total

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