Literature DB >> 9631037

In vivo EPR detection and imaging of endogenous nitric oxide in lipopolysaccharide-treated mice.

T Yoshimura1, H Yokoyama, S Fujii, F Takayama, K Oikawa, H Kamada.   

Abstract

Nitric oxide (NO), a simple diatomic free radical, is known to play a critical physiological role in diverse organisms. An iron complex, with N-(dithiocarboxy)sarcosine (Fe-DTCS), has a high affinity for endogenous NO and can trap, stabilize, and accumulate it. The stable NO adduct thus formed is detectable at room temperature with electron paramagnetic resonance (EPR) spectrometry. We report in vivo EPR imaging of endogenous NO, trapped by an Fe-DTCS complex, in the abdomen of a live mouse. To our knowledge, this is the first report on EPR imaging of endogenous free radicals produced in vivo. This EPR imaging method will be useful for the noninvasive investigation of the spatial distribution of NO in pathologic organs or tissues.

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Year:  1996        PMID： 9631037     DOI： 10.1038/nbt0896-992

Source DB:  PubMed          Journal:  Nat Biotechnol        ISSN： 1087-0156            Impact factor:   54.908


  20 in total

Review 1.  Physiological reactions of nitric oxide and hemoglobin: a radical rethink.

Authors:  S S Gross; P Lane
Journal:  Proc Natl Acad Sci U S A       Date:  1999-08-31       Impact factor: 11.205

Review 2.  Analytical chemistry of nitric oxide.

Authors:  Evan M Hetrick; Mark H Schoenfisch
Journal:  Annu Rev Anal Chem (Palo Alto Calif)       Date:  2009       Impact factor: 10.745

3.  Reaching the quantum limit of sensitivity in electron spin resonance.

Authors:  A Bienfait; J J Pla; Y Kubo; M Stern; X Zhou; C C Lo; C D Weis; T Schenkel; M L W Thewalt; D Vion; D Esteve; B Julsgaard; K Mølmer; J J L Morton; P Bertet
Journal:  Nat Nanotechnol       Date:  2015-12-14       Impact factor: 39.213

Review 4.  Use of electron paramagnetic resonance spectroscopy to evaluate the redox state in vivo.

Authors:  Harold M Swartz; Nadeem Khan; Valery V Khramtsov
Journal:  Antioxid Redox Signal       Date:  2007-10       Impact factor: 8.401

5.  Segmented surface coil resonator for in vivo EPR applications at 1.1GHz.

Authors:  Sergey Petryakov; Alexandre Samouilov; Michael Chzhan-Roytenberg; Eric Kesselring; Ziqi Sun; Jay L Zweier
Journal:  J Magn Reson       Date:  2008-12-24       Impact factor: 2.229

6.  Use of a hemoglobin-trapping approach in the determination of nitric oxide in in vitro and in vivo systems.

Authors:  A Balcioglu; C J Watkins; T J Maher
Journal:  Neurochem Res       Date:  1998-05       Impact factor: 3.996

Review 7.  Oxidative stress imaging in live animals with techniques based on electron paramagnetic resonance.

Authors:  Martyna Elas; Kazuhiro Ichikawa; Howard J Halpern
Journal:  Radiat Res       Date:  2012-02-21       Impact factor: 2.841

8.  In vivo detection of nitric oxide distribution in mice.

Authors:  Andrei M Komarov
Journal:  Mol Cell Biochem       Date:  2002 May-Jun       Impact factor: 3.396

9.  The role of thiol and nitrosothiol compounds in the nitric oxide-forming reactions of the iron-N-methyl-d-glucamine dithiocarbamate complex.

Authors:  Koichiro Tsuchiya; Kazuyoshi Kirima; Masanori Yoshizumi; Hitoshi Houchi; Toshiaki Tamaki; Ronald P Mason
Journal:  Biochem J       Date:  2002-11-01       Impact factor: 3.857

10.  Very-low-frequency electron paramagnetic resonance (EPR) imaging of nitroxide-loaded cells.

Authors:  Joseph P Y Kao; Eugene D Barth; Scott R Burks; Philip Smithback; Colin Mailer; Kang-Hyun Ahn; Howard J Halpern; Gerald M Rosen
Journal:  Magn Reson Med       Date:  2007-10       Impact factor: 4.668
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