Literature DB >> 962894

Differences between newly formed and recycled free small ribosome subunits in liver cytoplasm.

R H Hinton, B M Mullock.   

Abstract

Heterogeneity among the free small ribosomal subunits of rat liver was studied. Newly made free small subunits differ from the bulk of the free subunits in the following ways. (1) The newly made free small subunits sediment slightly faster than most free small subunits and form a band at slightly lower densities. (2) Mature free small subunits, prepared under conditions which minimize the amount of loosely bound protein, readily dimerize in solutions of low ionic strength. Little dimerization is found with newly formed subunits. This permits the separation of fractions enriched in newly formed subunits. (3) The fraction enriched in newly formed subunits contains a distinctive additional protein as compared the most of the free small subunits.

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Year:  1976        PMID: 962894      PMCID: PMC1163941          DOI: 10.1042/bj1580097

Source DB:  PubMed          Journal:  Biochem J        ISSN: 0264-6021            Impact factor:   3.857


  33 in total

1.  Comparison of proteins of ribosomal subunits and nucleolar preribosomal particles from Novikoff hepatoma ascites cells by two-dimensional polyacrylamide gel electrophoresis.

Authors:  A W Prestayko; G R Klomp; D J Schmoll; H Busch
Journal:  Biochemistry       Date:  1974-04-23       Impact factor: 3.162

2.  Two physically and functionally distinct forms of eukaryotic 40S ribosomal subunits.

Authors:  R E Wettenhall; I G Wool; C C Sherton
Journal:  Biochemistry       Date:  1973-06-19       Impact factor: 3.162

3.  Phosphorylation of proteins of ribosomes and nucleolar preribosomal particles from Novikoff hepatmoa ascites cells.

Authors:  M O Olson; A W Prestayko; C F Jones; H Busch
Journal:  J Mol Biol       Date:  1974-11-25       Impact factor: 5.469

4.  Isopycnic separation of subcellular components from poliovirus-infected and normal HeLa cells.

Authors:  D Baltimore; A S Huang
Journal:  Science       Date:  1968-11-01       Impact factor: 47.728

5.  Messenger RNA for globin in the postribosomal supernatant of rabbit reticulocytes.

Authors:  M Jacobs-Lorena; C Baglioni
Journal:  Proc Natl Acad Sci U S A       Date:  1972-06       Impact factor: 11.205

6.  Initiation of protein synthesis: evidence for messenger RNA-independent binding of methionyl-transfer RNA to the 40 S ribosomal subunit.

Authors:  C Darnbrough; S Legon; T Hunt; R J Jackson
Journal:  J Mol Biol       Date:  1973-05-25       Impact factor: 5.469

7.  Two separable cytoplasmic pools of native ribosomal subunits in chick embryo tissue culture cells.

Authors:  J L Hulse; F O Wettstein
Journal:  Biochim Biophys Acta       Date:  1972-05-10

8.  Molecular weight distribution of proteins in rabbit reticulocyte ribosomal subunits.

Authors:  H W King; H J Gould; J J Shearman
Journal:  J Mol Biol       Date:  1971-10-14       Impact factor: 5.469

9.  Messenger RNA-protein complexes and newly synthesized ribosomal subunits: analysis of free particles and components of polyribosomes.

Authors:  R P Perry; D E Kelley
Journal:  J Mol Biol       Date:  1968-07-14       Impact factor: 5.469

10.  Buoyant densities of cytoplasmic ribonucleoprotein particles of mammalian cells: distinctive character of ribosome subunits and the rapidly labeled components.

Authors:  R P Perry; D E Kelley
Journal:  J Mol Biol       Date:  1966-04       Impact factor: 5.469

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  1 in total

Review 1.  Heterogeneity of the translational machinery: Variations on a common theme.

Authors:  Martina Sauert; Hannes Temmel; Isabella Moll
Journal:  Biochimie       Date:  2014-12-24       Impact factor: 4.079

  1 in total

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