Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 The role of tetrahydrofolate dehydrogenase in the hepatic supply of tetrahydrobiopterin in rats.

Literature DB >> 962851

The role of tetrahydrofolate dehydrogenase in the hepatic supply of tetrahydrobiopterin in rats.

Abstract

The reduction of 7,8-dihydrobiopterin to 5,6,7,8-tetrahydrobiopterin by rat liver tetrahydrofolate dehydrogenase (5,6,7,8-tetrahydrofolate-NADP+ oxidoreductase, EC 1.5.1.3) is competitively inhibited by trimethoprim lactate (apparent Ki 0.285 muM). An apparent Michaelis constant of 43 muM for dihydrobiopterin was obtained, which is 430 times higher than the reported Km for dihydrofolate with this enzyme. The reduction of dihydrobiopterin is thus more susceptible to inhibition by trimethoprim lactate than is the reduction of dihydrofolate. However, intraperitoneal administration of trimethoprim had no significant effect on the hepatic supply of tetrahydrobiopterin in rats.

Entities: Chemical Species

Mesh：

Substances：

Year: 1976 PMID： 962851 PMCID： PMC1163821 DOI： 10.1042/bj1570105

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

14 in total

1. TYROSINE HYDROXYLASE. THE INITIAL STEP IN NOREPINEPHRINE BIOSYNTHESIS.

Authors: T NAGATSU; M LEVITT; S UDENFRIEND
Journal: J Biol Chem Date: 1964-09 Impact factor: 5.157

2. ELUCIDATION OF THE RATE-LIMITING STEP IN NOREPINEPHRINE BIOSYNTHESIS IN THE PERFUSED GUINEA-PIG HEART.

Authors: M LEVITT; S SPECTOR; A SJOERDSMA; S UDENFRIEND
Journal: J Pharmacol Exp Ther Date: 1965-04 Impact factor: 4.030

3. STUDIES ON THE STRUCTURE OF THE PRIMARY OXIDATION PRODUCT FORMED FROM TETRAHYDROPTERIDINES DURING PHENYLALAMINE HYDROXYLATION.

Authors: S KAUFMAN
Journal: J Biol Chem Date: 1964-01 Impact factor: 5.157

1. Biophysical characterization of full-length human phenylalanine hydroxylase provides a deeper understanding of its quaternary structure equilibrium.

Authors: Emilia C Arturo; Kushol Gupta; Michael R Hansen; Elias Borne; Eileen K Jaffe
Journal: J Biol Chem Date: 2019-05-10 Impact factor: 5.157

1 in total

The role of tetrahydrofolate dehydrogenase in the hepatic supply of tetrahydrobiopterin in rats.

1. TYROSINE HYDROXYLASE. THE INITIAL STEP IN NOREPINEPHRINE BIOSYNTHESIS.

2. ELUCIDATION OF THE RATE-LIMITING STEP IN NOREPINEPHRINE BIOSYNTHESIS IN THE PERFUSED GUINEA-PIG HEART.

3. STUDIES ON THE STRUCTURE OF THE PRIMARY OXIDATION PRODUCT FORMED FROM TETRAHYDROPTERIDINES DURING PHENYLALAMINE HYDROXYLATION.

4. Phenylalanine metabolism and folic acid antagonists.

5. Protein measurement with the Folin phenol reagent.

6. A soluble dihydrofolate reductase from human placenta: purification and properties.

7. Effect of co-trimoxazole on phenylalanine metabolism in man.

8. In vivo enhancement of tyrosine hydroxylation in rat striatum by tetrahydrobiopterin.

9. Letter: Tetrahydrobiopterin treatment of variant form of phenylketonuria.

10. The effect of L-ascorbate on catecholamine biosynthesis.

1. Biophysical characterization of full-length human phenylalanine hydroxylase provides a deeper understanding of its quaternary structure equilibrium.