Literature DB >> 9624421

A spin cartridge system for DNA extraction from paraffin wax embedded tissues.

A P Pinto1, L L Villa.   

Abstract

A simple and efficient method of DNA extraction from paraffin wax embedded tissues using a spin cartridge system is described. Such DNAs were shown to be suitable for amplification by the polymerase chain reaction, which targeted two human papillomavirus genes and one globin fragment giving rise to products of 450, 150, and 110 base pairs, respectively. Different human tissues, stored for up to 20 years, were successfully amplified, demonstrating the usefulness of this very simple procedure for retrospective studies.

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Year:  1998        PMID： 9624421      PMCID： PMC395609          DOI： 10.1136/mp.51.1.48

Source DB:  PubMed          Journal:  Mol Pathol        ISSN： 1366-8714


  6 in total

1.  Tissue extraction of DNA and RNA and analysis by the polymerase chain reaction.

Authors:  D P Jackson; F A Lewis; G R Taylor; A W Boylston; P Quirke
Journal:  J Clin Pathol       Date:  1990-06       Impact factor: 3.411

2.  The use of general primers GP5 and GP6 elongated at their 3' ends with adjacent highly conserved sequences improves human papillomavirus detection by PCR.

Authors:  A M de Roda Husman; J M Walboomers; A J van den Brule; C J Meijer; P J Snijders
Journal:  J Gen Virol       Date:  1995-04       Impact factor: 3.891

3.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors:  R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal:  Science       Date:  1988-01-29       Impact factor: 47.728

4.  Microwave-based DNA extraction from paraffin-embedded tissue for PCR amplification.

Authors:  S K Banerjee; W F Makdisi; A P Weston; S M Mitchell; D R Campbell
Journal:  Biotechniques       Date:  1995-05       Impact factor: 1.993

5.  Rapid preparation of tissue DNA from paraffin-embedded blocks and analysis by polymerase chain reaction.

Authors:  B F Chen; S Clejan
Journal:  J Histochem Cytochem       Date:  1993-05       Impact factor: 2.479

6.  A rapid and efficient method for DNA extraction from paraffin wax embedded tissue for PCR amplification.

Authors:  K Morgan; L Lam; N Kalsheker
Journal:  Clin Mol Pathol       Date:  1996-06
  6 in total
  2 in total

1.  Fatal pulmonary embolism: a study of genetic and acquired factors.

Authors:  K J Slovacek; A F Harris; J F Greene; A Rao
Journal:  Mol Diagn       Date:  2000-03

2.  Prevalence of human papillomavirus in archival samples obtained from patients with cervical pre-malignant and malignant lesions from Northeast Brazil.

Authors:  José V Fernandes; Rosely V Meissner; Maria Gf Carvalho; Thales Aam Fernandes; Paulo Rm Azevedo; João S Sobrinho; José Cm Prado; Luisa L Villa
Journal:  BMC Res Notes       Date:  2010-04-08
  2 in total

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