Literature DB >> 16696069

A rapid and efficient method for DNA extraction from paraffin wax embedded tissue for PCR amplification.

K Morgan1, L Lam, N Kalsheker.   

Abstract

DNA from archival, formaldehyde fixed, paraffin wax embedded human tissue, suitable for amplification by the polymerase chain reaction (PCR), was obtained using a microwave method based on the capture of DNA by magnetic beads. Fragments of the alpha-1-antitrypsin gene (AAT) and the apolipoprotein E gene (APOE) were amplified successfully from human liver and brain tissue, respectively. This procedure provides a more rapid, simple and efficient method for reproducibly obtaining DNA from preserved tissue that has been kept in storage for up to 30 years.

Entities:  

Year:  1996        PMID： 16696069      PMCID： PMC408047          DOI： 10.1136/mp.49.3.m179

Source DB:  PubMed          Journal:  Clin Mol Pathol        ISSN： 1355-2910


  5 in total

1.  Apolipoprotein E genotyping by one-stage PCR.

Authors:  P R Wenham; W H Price; G Blandell
Journal:  Lancet       Date:  1991-05-11       Impact factor: 79.321

2.  Microwave-based DNA extraction from paraffin-embedded tissue for PCR amplification.

Authors:  S K Banerjee; W F Makdisi; A P Weston; S M Mitchell; D R Campbell
Journal:  Biotechniques       Date:  1995-05       Impact factor: 1.993

3.  Rapid preparation of tissue DNA from paraffin-embedded blocks and analysis by polymerase chain reaction.

Authors:  B F Chen; S Clejan
Journal:  J Histochem Cytochem       Date:  1993-05       Impact factor: 2.479

4.  Analysis of DNA extracted from formalin-fixed, paraffin-embedded tissues by enzymatic amplification and hybridization with sequence-specific oligonucleotides.

Authors:  C C Impraim; R K Saiki; H A Erlich; R L Teplitz
Journal:  Biochem Biophys Res Commun       Date:  1987-02-13       Impact factor: 3.575

5.  Detection of human papilloma virus in paraffin-embedded tissue using the polymerase chain reaction.

Authors:  D K Shibata; N Arnheim; W J Martin
Journal:  J Exp Med       Date:  1988-01-01       Impact factor: 14.307
  5 in total
  4 in total

1.  DNA extraction from fresh-frozen and formalin-fixed, paraffin-embedded human brain tissue.

Authors:  Jian-Hua Wang; Amany Gouda-Vossos; Nicolas Dzamko; Glenda Halliday; Yue Huang
Journal:  Neurosci Bull       Date:  2013-08-30       Impact factor: 5.203

2.  A spin cartridge system for DNA extraction from paraffin wax embedded tissues.

Authors:  A P Pinto; L L Villa
Journal:  Mol Pathol       Date:  1998-02

3.  Association between CYP2C19 extensive metabolizer phenotype and childhood anti-reflux surgery following failed proton pump inhibitor medication treatment.

Authors:  James P Franciosi; Edward B Mougey; Andre Williams; Roberto A Gomez Suarez; Cameron Thomas; Christa L Creech; Katherine George; Diana Corao; John J Lima
Journal:  Eur J Pediatr       Date:  2017-12-06       Impact factor: 3.183

4.  Evaluation and Comparison of Genomic DNA Extraction Methods and PCR Optimization on Archival Formalin-Fixed and Paraffin-Embedded Tissues of Oral Squamous Cell Carcinoma.

Authors:  Samar Saeed Khan; Manisha Tijare; Sowmya Kasetty; Megha Jain; Ahmed Alamoudi; Hammam Ahmed Bahammam; Sarah Ahmed Bahammam; Maha A Bahammam; Saranya Varadarajan; A Thirumal Raj; Shankargouda Patil
Journal:  Diagnostics (Basel)       Date:  2022-05-12
  4 in total

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