Literature DB >> 9620426

Multiplex PCR for enterotoxigenic, attaching and effacing, and Shiga toxin-producing Escherichia coli strains from calves.

S M Franck1, B T Bosworth, H W Moon.   

Abstract

A multiplex PCR was developed to identify enterotoxigenic, attaching and effacing, and Shiga toxin-producing Escherichia coli strains by amplifying genes encoding K99 and F41 fimbriae, heat-stable enterotoxin a, intimin, and Shiga toxins 1 and 2. This multiplex PCR was specific and sensitive. It will be useful for identification of E. coli strains which cause diarrhea in calves.

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Year:  1998        PMID: 9620426      PMCID: PMC104926     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  25 in total

1.  Antigenic heterogeneity of Escherichia coli verotoxins.

Authors:  M A Karmali; M Petric; S Louie; R Cheung
Journal:  Lancet       Date:  1986-01-18       Impact factor: 79.321

2.  Nucleotide sequences of the genes for Escherichia coli heat-stable enterotoxin I of bovine, avian, and porcine origins.

Authors:  T Sekizaki; H Akashi; N Terakado
Journal:  Am J Vet Res       Date:  1985-04       Impact factor: 1.156

3.  Distribution and virulence of Escherichia coli in the small intestines of calves with and without diarrhea.

Authors:  R E Isaacson; H W Moon; R A Schneider
Journal:  Am J Vet Res       Date:  1978-11       Impact factor: 1.156

4.  Adhesion in vitro and in vivo associated with an adhesive antigen (F41) produced by a K99 mutant of the reference strain Escherichia coli B41.

Authors:  J A Morris; C Thorns; A C Scott; W J Sojka; G A Wells
Journal:  Infect Immun       Date:  1982-06       Impact factor: 3.441

5.  The establishment of K99, a thermolabile, transmissible escherichia coli K antigen, previously called "Kco", possessed by calf and lamb enteropathogenic strains.

Authors:  I Orskov; F Orskov; H W Smith; W J Sojka
Journal:  Acta Pathol Microbiol Scand B       Date:  1975-02

6.  Occurrence of enterotoxigenic Escherichia coli in calves with acute neonatal diarrhoea.

Authors:  H V Krogh
Journal:  Nord Vet Med       Date:  1983-10

7.  A clinicopathologic study of enterocyte-adherent Escherichia coli: a cause of protracted diarrhea in infants.

Authors:  R Rothbaum; A J McAdams; R Giannella; J C Partin
Journal:  Gastroenterology       Date:  1982-08       Impact factor: 22.682

8.  Biochemical characteristics of enterotoxigenic and nonenterotoxigenic Escherichia coli isolated from calves with diarrhea.

Authors:  B A Braaten; L L Myers
Journal:  Am J Vet Res       Date:  1977-12       Impact factor: 1.156

9.  Diarrhea due to Escherichia coli strain RDEC-1 in the rabbit: the peyer's patch as the initial site of attachment and colonization.

Authors:  J R Cantey; L R Inman
Journal:  J Infect Dis       Date:  1981-03       Impact factor: 5.226

10.  Attaching and effacing activities of rabbit and human enteropathogenic Escherichia coli in pig and rabbit intestines.

Authors:  H W Moon; S C Whipp; R A Argenzio; M M Levine; R A Giannella
Journal:  Infect Immun       Date:  1983-09       Impact factor: 3.441

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  59 in total

1.  Identification of bacterial populations in dairy wastewaters by use of 16S rRNA gene sequences and other genetic markers.

Authors:  Jeffery A McGarvey; William G Miller; Susan Sanchez; Larry Stanker
Journal:  Appl Environ Microbiol       Date:  2004-07       Impact factor: 4.792

2.  In vivo transduction of an Stx-encoding phage in ruminants.

Authors:  Nancy A Cornick; Amy F Helgerson; Volker Mai; Jennifer M Ritchie; David W K Acheson
Journal:  Appl Environ Microbiol       Date:  2006-07       Impact factor: 4.792

3.  Shiga Toxin-Producing E. coli in Animals: Detection, Characterization, and Virulence Assessment.

Authors:  Stefanie A Barth; Rolf Bauerfeind; Christian Berens; Christian Menge
Journal:  Methods Mol Biol       Date:  2021

4.  Identification of virulence factors by multiplex PCR in Escherichia coli isolated from calves in Minas Gerais, Brazil.

Authors:  Giovanna I Andrade; Fernanda M Coura; Ethiene L S Santos; Marina G Ferreira; Grazielle C F Galinari; Elias J Facury Filho; Antônio U de Carvalho; Andrey P Lage; Marcos B Heinemann
Journal:  Trop Anim Health Prod       Date:  2012-04-04       Impact factor: 1.559

5.  Isolation of Escherichia coli from piglets in South Korea with diarrhea and characteristics of the virulence genes.

Authors:  Yeong Ju Kim; Ji Hee Kim; Jin Hur; John Hwa Lee
Journal:  Can J Vet Res       Date:  2010-01       Impact factor: 1.310

6.  Molecular detection of virulence genes and multi-drug resistance patterns in Escherichia coli (STEC) in clinical bovine mastitis: Alborz province, Iran.

Authors:  M Tavakoli; H Pourtaghi
Journal:  Iran J Vet Res       Date:  2017       Impact factor: 1.376

7.  Differences in levels of secreted locus of enterocyte effacement proteins between human disease-associated and bovine Escherichia coli O157.

Authors:  A McNally; A J Roe; S Simpson; F M Thomson-Carter; D E Hoey; C Currie; T Chakraborty; D G Smith; D L Gally
Journal:  Infect Immun       Date:  2001-08       Impact factor: 3.441

8.  Evaluation of broiler litter with reference to the microbial composition as assessed by using 16S rRNA and functional gene markers.

Authors:  Jingrang Lu; Susan Sanchez; Charles Hofacre; John J Maurer; Barry G Harmon; Margie D Lee
Journal:  Appl Environ Microbiol       Date:  2003-02       Impact factor: 4.792

9.  Simultaneous Screening of 24 Target Genes of Foodborne Pathogens in 35 Foodborne Outbreaks Using Multiplex Real-Time SYBR Green PCR Analysis.

Authors:  Hiroshi Fukushima; Jun Kawase; Yoshiki Etoh; Kumiko Sugama; Shunshuke Yashiro; Natsuko Iida; Keiji Yamaguchi
Journal:  Int J Microbiol       Date:  2010-09-28

10.  Comprehensive and rapid real-time PCR analysis of 21 foodborne outbreaks.

Authors:  Hiroshi Fukushima; Kazunori Katsube; Yoshie Tsunomori; Ryoko Kishi; Junko Atsuta; Yuko Akiba
Journal:  Int J Microbiol       Date:  2009-06-24
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