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Literature DB >> 9611254

Use of capillary electrophoresis in the study of ligand-DNA interactions.

Abstract

Free solution capillary electrophoresis (FSCE) has been used to separate two non-self-complementary 12mer oligonucleotide duplexes: d(AAATTATATTAT).d(ATAA-TATAATTT) and d(GGGCCGCGCCGC).d(GCGGCGCGGCCC). Titration of mixtures of the two oligonucleotides with model intercalators (ethidium bromide andactinomycin D) and minor groove binders (netropsin, Hoechst 33258 and distamycin) has shown the suitability of FSCE as a method to study the sequence selectivity of DNA binding agents. Binding data have shown cooperativity of binding for netropsin and Hoechst 33258 and have provided ligand:DNA binding ratios for all five compounds. Cooperativity of netropsin binding to a 12mer with two potential sites has been demonstrated for the first time. Ligands binding in the minor groove caused changes in migration time and peak shape which were significantly different from those caused by intercalators.

Entities: Chemical

Mesh：

Substances：

Year: 1998 PMID： 9611254 PMCID： PMC147652 DOI： 10.1093/nar/26.12.3053

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

26 in total

1. Evidence for sequence preferences in the intercalative binding of ethidium bromide to dinucleoside monophosphates.

Authors: T R Krugh; C G Reinhardt
Journal: J Mol Biol Date: 1975-09-15 Impact factor: 5.469

2. DNAse footprinting: a simple method for the detection of protein-DNA binding specificity.

Authors: D J Galas; A Schmitz
Journal: Nucleic Acids Res Date: 1978-09 Impact factor: 16.971

3. Separation of pd(GC)12 from pd(AT)12 by free solution capillary electrophoresis.

Authors: I I Hamdan; G G Skellern; R D Waigh
Journal: J Chromatogr A Date: 1998-05-08 Impact factor: 4.759

4. Footprinting at low temperatures: evidence that ethidium and other simple intercalators can discriminate between different nucleotide sequences.

Authors: K R Fox; M J Waring
Journal: Nucleic Acids Res Date: 1987-01-26 Impact factor: 16.971

Review 5. Spectroscopic analysis of drug-nucleic acid interactions.

Authors: G Dougherty; W J Pigram
Journal: CRC Crit Rev Biochem Date: 1982-02

6. Sequence specificity of actinomycin D and Netropsin binding to pBR322 DNA analyzed by protection from DNase I.

Authors: M J Lane; J C Dabrowiak; J N Vournakis
Journal: Proc Natl Acad Sci U S A Date: 1983-06 Impact factor: 11.205

7. Conformation dependent binding of netropsin and distamycin to DNA and DNA model polymers.

Authors: G Luck; H Triebel; M Waring; C Zimmer
Journal: Nucleic Acids Res Date: 1974-03 Impact factor: 16.971

8. Map of distamycin, netropsin, and actinomycin binding sites on heterogeneous DNA: DNA cleavage-inhibition patterns with methidiumpropyl-EDTA.Fe(II).

Authors: M W Van Dyke; R P Hertzberg; P B Dervan
Journal: Proc Natl Acad Sci U S A Date: 1982-09 Impact factor: 11.205

9. 1H NMR studies on the interaction between distamycin A and a symmetrical DNA dodecamer.

Authors: R E Klevit; D E Wemmer; B R Reid
Journal: Biochemistry Date: 1986-06-03 Impact factor: 3.162

10. DNA sequence preferences for the anti-cancer drug mitoxanthrone and related anthraquinones revealed by DNase I footprinting.

Authors: K R Fox; M J Waring; J R Brown; S Neidle
Journal: FEBS Lett Date: 1986-07-07 Impact factor: 4.124

5 in total

1. Sequence-dependent variation in DNA minor groove width dictates orientational preference of Hoechst 33258 in A-tract recognition: solution NMR structure of the 2:1 complex with d(CTTTTGCAAAAG)(2).

Authors: E Gavathiotis; G J Sharman; M S Searle
Journal: Nucleic Acids Res Date: 2000-02-01 Impact factor: 16.971

2. Massive parallel analysis of the binding specificity of histone-like protein HU to single- and double-stranded DNA with generic oligodeoxyribonucleotide microchips.

Authors: A S Krylov; O A Zasedateleva; D V Prokopenko; J Rouviere-Yaniv; A D Mirzabekov
Journal: Nucleic Acids Res Date: 2001-06-15 Impact factor: 16.971