Literature DB >> 19100556

Effect of the matrix on DNA electrophoretic mobility.

Nancy C Stellwagen1, Earle Stellwagen.   

Abstract

DNA electrophoretic mobilities are highly dependent on the nature of the matrix in which the separation takes place. This review describes the effect of the matrix on DNA separations in agarose gels, polyacrylamide gels and solutions containing entangled linear polymers, correlating the electrophoretic mobilities with information obtained from other types of studies. DNA mobilities in various sieving media are determined by the interplay of three factors: the relative size of the DNA molecule with respect to the effective pore size of the matrix, the effect of the electric field on the matrix, and specific interactions of DNA with the matrix during electrophoresis.

Entities:  

Mesh：

Substances：

Year:  2008        PMID： 19100556      PMCID： PMC2643323          DOI： 10.1016/j.chroma.2008.11.090

Source DB:  PubMed          Journal:  J Chromatogr A        ISSN： 0021-9673            Impact factor:   4.759


  181 in total

1.  Three-dimensional observation of electrophoretic migration of dsDNA in semidilute hydroxyethylcellulose solution.

Authors:  O de Carmejane; Y Yamaguchi; T I Todorov; M D Morris
Journal:  Electrophoresis       Date:  2001-08       Impact factor: 3.535

2.  Determining the electrophoretic mobility and translational diffusion coefficients of DNA molecules in free solution.

Authors:  Earle Stellwagen; Nancy C Stellwagen
Journal:  Electrophoresis       Date:  2002-08       Impact factor: 3.535

3.  Simultaneous measurements of the electrophoretic mobility, diffusion coefficient and orientation of dsDNA during electrophoresis in polymer solutions.

Authors:  Axel Ekani Nkodo; Bernard Tinland
Journal:  Electrophoresis       Date:  2002-08       Impact factor: 3.535

4.  Molecular detrapping and band narrowing with high frequency modulation of pulsed field electrophoresis.

Authors:  C Turmel; E Brassard; G W Slater; J Noolandi
Journal:  Nucleic Acids Res       Date:  1990-02-11       Impact factor: 16.971

5.  Transient orientation of linear DNA molecules during pulsed-field gel electrophoresis.

Authors:  G Holzwarth; C B McKee; S Steiger; G Crater
Journal:  Nucleic Acids Res       Date:  1987-12-10       Impact factor: 16.971

6.  Tertiary and quaternary structure in aqueous polysaccharide systems which model cell wall cohesion: reversible changes in conformation and association of agarose, carrageenan and galactomannans.

Authors:  I C Dea; A A McKinnon; D A Rees
Journal:  J Mol Biol       Date:  1972-07-14       Impact factor: 5.469

7.  Properties of supercoiled DNA in gel electrophoresis. The V-like dependence of mobility on topological constraint. DNA-matrix interactions.

Authors:  Y Zivanovic; I Goulet; A Prunell
Journal:  J Mol Biol       Date:  1986-12-05       Impact factor: 5.469

8.  Measurement of DNA length by gel electrophoresis.

Authors:  E M Southern
Journal:  Anal Biochem       Date:  1979-12       Impact factor: 3.365

9.  Electrophoretic mobility is a reporter of hairpin structure in single-stranded DNA oligomers.

Authors:  Earle Stellwagen; Arian Abdulla; Qian Dong; Nancy C Stellwagen
Journal:  Biochemistry       Date:  2007-09-01       Impact factor: 3.162

10.  Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

Authors:  D C Schwartz; C R Cantor
Journal:  Cell       Date:  1984-05       Impact factor: 41.582
View more
  12 in total

1.  Topological patterns in two-dimensional gel electrophoresis of DNA knots.

Authors:  Davide Michieletto; Davide Marenduzzo; Enzo Orlandini
Journal:  Proc Natl Acad Sci U S A       Date:  2015-09-08       Impact factor: 11.205

2.  Simultaneous RNA purification and size selection using on-chip isotachophoresis with an ionic spacer.

Authors:  Crystal M Han; David Catoe; Sarah A Munro; Ruba Khnouf; Michael P Snyder; Juan G Santiago; Marc L Salit; Can Cenik
Journal:  Lab Chip       Date:  2019-07-22       Impact factor: 6.799

Review 3.  Electrophoresis of DNA in agarose gels, polyacrylamide gels and in free solution.

Authors:  Nancy C Stellwagen
Journal:  Electrophoresis       Date:  2009-06       Impact factor: 3.535

4.  Probing biomolecular structures and dynamics of single molecules using in-gel alternating-laser excitation.

Authors:  Yusdi Santoso; Achillefs N Kapanidis
Journal:  Anal Chem       Date:  2009-12-01       Impact factor: 6.986

5.  Regulation of cell attachment, spreading, and migration by hydrogel substrates with independently tunable mesh size.

Authors:  Jing Xia; Zong-Yuan Liu; Zheng-Yuan Han; Yuan Yuan; Yue Shao; Xi-Qiao Feng; David A Weitz
Journal:  Acta Biomater       Date:  2022-01-15       Impact factor: 8.947

6.  HIV detection from human serum with paper-based isotachophoretic RNA extraction and reverse transcription recombinase polymerase amplification.

Authors:  Andrew T Bender; Benjamin P Sullivan; Jane Y Zhang; David C Juergens; Lorraine Lillis; David S Boyle; Jonathan D Posner
Journal:  Analyst       Date:  2021-05-04       Impact factor: 5.227

7.  Force fields of charged particles in micro-nanofluidic preconcentration systems.

Authors:  Lingyan Gong; Wei Ouyang; Zirui Li; Jongyoon Han
Journal:  AIP Adv       Date:  2017-12-21       Impact factor: 1.548

8.  A novel 4-arm DNA/RNA Nanoconstruct triggering Rapid Apoptosis of Triple Negative Breast Cancer Cells within 24 hours.

Authors:  Joline Tung; Lih Shin Tew; Yuan-Man Hsu; Yit Lung Khung
Journal:  Sci Rep       Date:  2017-04-11       Impact factor: 4.379

9.  Electrode-Free Concentration and Recovery of DNA at Physiologically Relevant Ionic Concentrations.

Authors:  Sixuan Li; Andrew Li; Kuangwen Hsieh; Sarah M Friedrich; Tza-Huei Wang
Journal:  Anal Chem       Date:  2020-04-13       Impact factor: 8.008

10.  Palindromic genes in the linear mitochondrial genome of the nonphotosynthetic green alga Polytomella magna.

Authors:  David Roy Smith; Jimeng Hua; John M Archibald; Robert W Lee
Journal:  Genome Biol Evol       Date:  2013       Impact factor: 3.416
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.