Literature DB >> 9603851

Method detection limits of PCR and immunofluorescence assay for Cryptosporidium parvum in soil.

M J Walker1, C Montemagno, J C Bryant, W C Ghiorse.   

Abstract

We determined and compared the method detection limits (MDLalpha) of a PCR and an immunofluorescence assay (IFA) for detection of Cryptosporidium parvum oocysts in soils. Based on the MDLalpha and the quantitative nature and stability of the IFA, PCR analysis is not a useful screening step for soil studies of oocyst transport.

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Year:  1998        PMID： 9603851      PMCID： PMC106315     

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  4 in total

1.  Assessment of a dye permeability assay for determination of inactivation rates of Cryptosporidium parvum oocysts.

Authors:  M B Jenkins; L J Anguish; D D Bowman; M J Walker; W C Ghiorse
Journal:  Appl Environ Microbiol       Date:  1997-10       Impact factor: 4.792

2.  Computer-Assisted Laser Scanning and Video Microscopy for Analysis of Cryptosporidium parvum Oocysts in Soil, Sediment, and Feces.

Authors:  L J Anguish; W C Ghiorse
Journal:  Appl Environ Microbiol       Date:  1997-02       Impact factor: 4.792

3.  Evaluation of PCR, nested PCR, and fluorescent antibodies for detection of Giardia and Cryptosporidium species in wastewater.

Authors:  C L Mayer; C J Palmer
Journal:  Appl Environ Microbiol       Date:  1996-06       Impact factor: 4.792

4.  Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples.

Authors:  D W Johnson; N J Pieniazek; D W Griffin; L Misener; J B Rose
Journal:  Appl Environ Microbiol       Date:  1995-11       Impact factor: 4.792
  4 in total
  4 in total

1.  Use of a sentinel system for field measurements of Cryptosporidium parvum oocyst inactivation in soil and animal waste.

Authors:  M B Jenkins; M J Walker; D D Bowman; L C Anthony; W C Ghiorse
Journal:  Appl Environ Microbiol       Date:  1999-05       Impact factor: 4.792

2.  Detection of Cryptosporidium parvum in soil extracts.

Authors:  Mark Walker; Douglas Redelman
Journal:  Appl Environ Microbiol       Date:  2004-03       Impact factor: 4.792

3.  Method for detection and enumeration of Cryptosporidium parvum oocysts in feces, manures, and soils.

Authors:  E Kuczynska; D R Shelton
Journal:  Appl Environ Microbiol       Date:  1999-07       Impact factor: 4.792

4.  Immunofluorescence assay and PCR analysis of cryptosporidium oocysts and species from human feacal specimens.

Authors:  Mehdi Vejdani; Rezaei Mansour; Yezdan Hamzavi; Sina Vejdani; Naser Nazeri; Ali Michaeli
Journal:  Jundishapur J Microbiol       Date:  2014-06-01       Impact factor: 0.747
  4 in total

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