Literature DB >> 15006810

Detection of Cryptosporidium parvum in soil extracts.

Mark Walker1, Douglas Redelman.   

Abstract

Epifluorescent microscopy and flow cytometry were used in different combinations with fluorescein isothiocyanate-labeled immunoglobulins M and G3 to estimate the numbers of Cryptosporidium parvum oocysts in soil extracts containing 10 to 10,017 oocysts/ml. No combination had a systematic effect on accuracy or precision. Background debris may have produced overestimates at low oocyst concentrations when flow cytometry was used.

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Year:  2004        PMID： 15006810      PMCID： PMC368368          DOI： 10.1128/AEM.70.3.1827-1829.2004

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  5 in total

1.  A novel two-color flow cytometric assay for the detection of Cryptosporidium in environmental water samples.

Authors:  B C Ferrari; G Vesey; K A Davis; M Gauci; D Veal
Journal:  Cytometry       Date:  2000-11-01

2.  Method detection limits of PCR and immunofluorescence assay for Cryptosporidium parvum in soil.

Authors:  M J Walker; C Montemagno; J C Bryant; W C Ghiorse
Journal:  Appl Environ Microbiol       Date:  1998-06       Impact factor: 4.792

3.  Continuous-flow differential density flotation of coccidial oocysts and a comparison with other methods.

Authors:  J M Vetterling
Journal:  J Parasitol       Date:  1969-04       Impact factor: 1.276

4.  Effects of combined water potential and temperature stresses on Cryptosporidium parvum oocysts.

Authors:  M Walker; K Leddy; E Hager; E Hagar
Journal:  Appl Environ Microbiol       Date:  2001-12       Impact factor: 4.792

5.  Comparison of conventional staining methods and monoclonal antibody-based methods for Cryptosporidium oocyst detection.

Authors:  M J Arrowood; C R Sterling
Journal:  J Clin Microbiol       Date:  1989-07       Impact factor: 5.948
  5 in total

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