Literature DB >> 9600927

Huntingtin aggregation monitored by dynamic light scattering.

Y Georgalis1, E B Starikov, B Hollenbach, R Lurz, E Scherzinger, W Saenger, H Lehrach, E E Wanker.   

Abstract

An initial stage of fibrillogenesis in solutions of glutathione S-transferase-huntingtin (GST-HD) fusion proteins has been studied by using dynamic light scattering. Two GST-HD systems with poly-L-glutamine (polyGln) extensions of different lengths (20 and 51 residues) have been examined. For both systems, kinetics of z-average translation diffusion coefficients (Dapp) and their angular dependence have been obtained. Our data reveal that aggregation does occur in both GST-HD51 and GST-HD20 solutions, but that it is much more pronounced in the former. Thus, our approach provides a powerful tool for the quantitative assay of GST-HD fibrillogenesis in vitro.

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Year:  1998        PMID： 9600927      PMCID： PMC27595          DOI： 10.1073/pnas.95.11.6118

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  15 in total

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Journal:  Am J Hum Genet       Date:  1996-07       Impact factor: 11.025

3.  Kinetic theory of fibrillogenesis of amyloid beta-protein.

Authors:  A Lomakin; D B Teplow; D A Kirschner; G B Benedek
Journal:  Proc Natl Acad Sci U S A       Date:  1997-07-22       Impact factor: 11.205

4.  Identification of an HD patient with a (CAG)180 repeat expansion and the propagation of highly expanded CAG repeats in lambda phage.

Authors:  K Sathasivam; I Amaechi; L Mangiarini; G Bates
Journal:  Hum Genet       Date:  1997-05       Impact factor: 4.132

Review 5.  Models of amyloid seeding in Alzheimer's disease and scrapie: mechanistic truths and physiological consequences of the time-dependent solubility of amyloid proteins.

Authors:  J D Harper; P T Lansbury
Journal:  Annu Rev Biochem       Date:  1997       Impact factor: 23.643

Review 6.  Glutamine repeats and inherited neurodegenerative diseases: molecular aspects.

Authors:  M F Perutz
Journal:  Curr Opin Struct Biol       Date:  1996-12       Impact factor: 6.809

7.  Huntingtin-encoded polyglutamine expansions form amyloid-like protein aggregates in vitro and in vivo.

Authors:  E Scherzinger; R Lurz; M Turmaine; L Mangiarini; B Hollenbach; R Hasenbank; G P Bates; S W Davies; H Lehrach; E E Wanker
Journal:  Cell       Date:  1997-08-08       Impact factor: 41.582

8.  Formation of neuronal intranuclear inclusions underlies the neurological dysfunction in mice transgenic for the HD mutation.

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  20 in total

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Journal:  Anal Chem       Date:  2011-09-22       Impact factor: 6.986

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7.  Diffusive and directional intracellular dynamics measured by field-based dynamic light scattering.

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8.  Generation of neuronal intranuclear inclusions by polyglutamine-GFP: analysis of inclusion clearance and toxicity as a function of polyglutamine length.

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9.  Study of the aggregation mechanism of polyglutamine peptides using replica exchange molecular dynamics simulations.

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Journal:  Proc Natl Acad Sci U S A       Date:  2003-11-14       Impact factor: 11.205
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