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Literature DB >> 9598716

A simple and efficient method for microdissection and microFISH.

J J Engelen¹, J C Albrechts, G J Hamers, J P Geraedts.

Abstract

A simple and efficient method for the dissection of (marker) chromosomes, (micro)nuclei, and chromosome regions is presented. Before microdissection, metaphases are overlaid with milli-Q water to rehydrate the chromosomes, which makes them soft and sticky. The dissected chromosome fragments are dissolved without proteinase-K or topoisomerase treatment and directly amplified using a degenerate oligonucleotide primed polymerase chain reaction (DOP-PCR). The advantages of this microFISH method over previously reported methods are: (1) microdissection in this way is very fast; (2) a chromosome, marker, (micro)nucleus, or chromosome region is collected as a whole using only one microneedle; (3) the dissected material sticks tightly to the needle without the risk of getting lost; (4) no Sequenase is used in the DOP-PCR reaction which reduces the risk of contamination.

Entities: Chemical

Mesh：

Year: 1998 PMID： 9598716 PMCID： PMC1051270 DOI： 10.1136/jmg.35.4.265

Source DB: PubMed Journal: J Med Genet ISSN： 0022-2593 Impact factor: 6.318

16 in total

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Authors: P S Meltzer; X Y Guan; A Burgess; J M Trent
Journal: Nat Genet Date: 1992-04 Impact factor: 38.330

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Journal: Hum Genet Date: 1992-04 Impact factor: 4.132

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Authors: J J Engelen; W J Loots; J C Albrechts; P C Motoh; J P Fryns; A J Hamers; J P Geraedts
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Authors: X Y Guan; J M Trent; P S Meltzer
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Authors: U Claussen; A Mazur; N Rubtsov
Journal: Cytogenet Cell Genet Date: 1994

8. High primer concentration improves PCR amplification from random pools.

Authors: T Czerny
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9. Chromosome microdissection identifies cryptic sites of DNA sequence amplification in human ovarian carcinoma.

Authors: X Y Guan; C B Cargile; S L Anzick; F H Thompson; P S Meltzer; M L Bittner; R Taetle; J R McGill; J M Trent
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Review 10. ICF syndrome: a new case and review of the literature.

Authors: D F Smeets; U Moog; C M Weemaes; G Vaes-Peeters; G F Merkx; J P Niehof; G Hamers
Journal: Hum Genet Date: 1994-09 Impact factor: 4.132

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3. Formation of novel CENP-A domains on tandem repetitive DNA and across chromosome breakpoints on human chromosome 8q21 neocentromeres.

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4. Design and validation of a pericentromeric BAC clone set aimed at improving diagnosis and phenotype prediction of supernumerary marker chromosomes.

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Journal: Mol Cytogenet Date: 2013-10-30 Impact factor: 2.009

5. Microdissection of the A_h01 chromosome in upland cotton and microcloning of resistance gene anologs from the single chromosome.

Authors: Xinchuan Cao; Yuling Liu; Zhen Liu; Fang Liu; Yalei Wu; Zhongli Zhou; Xiaoyan Cai; Xingxing Wang; Zhenmei Zhang; Yuhong Wang; Zhimin Luo; Renhai Peng; Kunbo Wang
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6. Evaluation of the reliability of chromosomal imbalances detected by combined use of universal DNA amplification and comparative genomic hybridization.

Authors: T Harada; K Shiraishi; N Kusano; K Umayahara; S Kondoh; K Okita; K Sasaki
Journal: Jpn J Cancer Res Date: 2000-11

7. Clinical outcomes following preimplantation genetic testing and microdissecting junction region in couples with balanced chromosome rearrangement.

Authors: Dehua Cheng; Liang Hu; Fei Gong; Shimin Yuan; Keli Luo; Xianhong Wu; Pingyuan Xie; Changfu Lu; Guangxiu Lu; Yue-Qiu Tan; Ge Lin
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7 in total