Dehua Cheng1, Liang Hu1,2,3,4,5, Fei Gong1,2,3,4,5, Shimin Yuan1, Keli Luo1,2, Xianhong Wu3, Pingyuan Xie3, Changfu Lu1,2,3,4, Guangxiu Lu1,2,3,4,5, Yue-Qiu Tan6,7,8,9, Ge Lin10,11,12,13,14. 1. Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, Hunan, China. 2. Institute of Reproduction and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, Hunan, China. 3. National Engineering and Research Center of Human Stem Cells, Changsha, Hunan, China. 4. NHC Key Laboratory of Human Stem Cell and Reproductive Engineering, Central South University, Changsha, Hunan, China. 5. Clinical Research Center for Reproduction and Genetics in Hunan Province, Changsha, Hunan, China. 6. Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, Hunan, China. yueqiutan2019@163.com. 7. Institute of Reproduction and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, Hunan, China. yueqiutan2019@163.com. 8. NHC Key Laboratory of Human Stem Cell and Reproductive Engineering, Central South University, Changsha, Hunan, China. yueqiutan2019@163.com. 9. Clinical Research Center for Reproduction and Genetics in Hunan Province, Changsha, Hunan, China. yueqiutan2019@163.com. 10. Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, Hunan, China. linggf@hotmail.com. 11. Institute of Reproduction and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, Hunan, China. linggf@hotmail.com. 12. National Engineering and Research Center of Human Stem Cells, Changsha, Hunan, China. linggf@hotmail.com. 13. NHC Key Laboratory of Human Stem Cell and Reproductive Engineering, Central South University, Changsha, Hunan, China. linggf@hotmail.com. 14. Clinical Research Center for Reproduction and Genetics in Hunan Province, Changsha, Hunan, China. linggf@hotmail.com.
Abstract
PURPOSE: The purpose of this study is to summarize the clinical outcomes of apparently balanced chromosome rearrangement (ABCR) carriers in preimplantation genetic testing (PGT) cycles by next-generation sequencing following microdissecting junction region (MicroSeq) to distinguish non-carrier embryos from balanced carriers. METHODS: A retrospective study of 762 ABCR carrier couples who requested PGT for structural rearrangements combined with MicroSeq at the Reproductive and Genetic Hospital of CITIC-Xiangya was conducted between October 2014 and October 2019. RESULTS: Trophectoderm biopsy was performed in 4122 blastocysts derived from 917 PGT-SR cycles and 3781 blastocysts were detected. Among the 3781 blastocysts diagnosed, 1433 (37.9%, 1433/3781) were balanced, of which 739 blastocysts were carriers (51.57%, 739/1433) and 694 blastocysts were normal (48.43%, 694/1433). Approximately 26.39% of cycles had both carrier and normal embryo transfer, and the average number of biopsied blastocysts was 6.7. In the cumulative 223 biopsied cycles with normal embryo transfer, all couples chose to transfer the normal embryos. In the 225 cycles with only carrier embryos, the couples chose to transfer the carrier embryos in 169/225 (75.11%) cycles. A total of 732 frozen embryo transfer cycles were performed, resulting in 502 clinical pregnancies. Cumulatively, 326 babies were born; all of these babies were healthy and free of any developmental issues. CONCLUSION: Our study provides the first evaluation of the clinical outcomes of a large sample with ABCR carrier couples undergoing the MicroSeq-PGT technique and reveals its powerful ability to distinguish between carrier and non-carrier balanced embryos.
PURPOSE: The purpose of this study is to summarize the clinical outcomes of apparently balanced chromosome rearrangement (ABCR) carriers in preimplantation genetic testing (PGT) cycles by next-generation sequencing following microdissecting junction region (MicroSeq) to distinguish non-carrier embryos from balanced carriers. METHODS: A retrospective study of 762 ABCR carrier couples who requested PGT for structural rearrangements combined with MicroSeq at the Reproductive and Genetic Hospital of CITIC-Xiangya was conducted between October 2014 and October 2019. RESULTS: Trophectoderm biopsy was performed in 4122 blastocysts derived from 917 PGT-SR cycles and 3781 blastocysts were detected. Among the 3781 blastocysts diagnosed, 1433 (37.9%, 1433/3781) were balanced, of which 739 blastocysts were carriers (51.57%, 739/1433) and 694 blastocysts were normal (48.43%, 694/1433). Approximately 26.39% of cycles had both carrier and normal embryo transfer, and the average number of biopsied blastocysts was 6.7. In the cumulative 223 biopsied cycles with normal embryo transfer, all couples chose to transfer the normal embryos. In the 225 cycles with only carrier embryos, the couples chose to transfer the carrier embryos in 169/225 (75.11%) cycles. A total of 732 frozen embryo transfer cycles were performed, resulting in 502 clinical pregnancies. Cumulatively, 326 babies were born; all of these babies were healthy and free of any developmental issues. CONCLUSION: Our study provides the first evaluation of the clinical outcomes of a large sample with ABCR carrier couples undergoing the MicroSeq-PGT technique and reveals its powerful ability to distinguish between carrier and non-carrier balanced embryos.
Authors: Nathan R Treff; Xin Tao; Wendy J Schillings; Paul A Bergh; Richard T Scott; Brynn Levy Journal: Fertil Steril Date: 2011-05-14 Impact factor: 7.329
Authors: Maureen T M Franssen; Johanna C Korevaar; Fulco van der Veen; Nico J Leschot; Patrick M M Bossuyt; Mariette Goddijn Journal: BMJ Date: 2006-02-22